Research output: Contribution to journal › Article › peer-review
Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer. / Kruchinina, M. V.; Prudnikova, Ya I.; Kurilovich, S. A. et al.
In: Siberian Journal of Oncology, Vol. 16, No. 4, 2017, p. 32-41.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer
AU - Kruchinina, M. V.
AU - Prudnikova, Ya I.
AU - Kurilovich, S. A.
AU - Gromov, A. A.
AU - Kruchinin, V. N.
AU - Atuchin, V. V.
AU - Naumova, O. V.
AU - Spesivtsev, E. V.
AU - Volodin, V. A.
AU - Peltek, S. E.
AU - Shuvalov, G. V.
AU - Generalov, V. M.
PY - 2017
Y1 - 2017
N2 - Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.
AB - Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.
KW - Colorectal cancer
KW - Diagnosis
KW - Ellipsometry
KW - Fluorescence optical spectrometry
KW - Raman spectroscopy
KW - SOI nanowire biosensor
KW - Surface Plasmon resonance
KW - Tumor M2 pyruvate kinase
UR - http://www.scopus.com/inward/record.url?scp=85041611340&partnerID=8YFLogxK
U2 - 10.21294/1814-4861-2017-16-4-32-41
DO - 10.21294/1814-4861-2017-16-4-32-41
M3 - Article
AN - SCOPUS:85041611340
VL - 16
SP - 32
EP - 41
JO - Siberian Journal of Oncology
JF - Siberian Journal of Oncology
SN - 1814-4861
IS - 4
ER -
ID: 9640595