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Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer. / Kruchinina, M. V.; Prudnikova, Ya I.; Kurilovich, S. A. et al.

In: Siberian Journal of Oncology, Vol. 16, No. 4, 2017, p. 32-41.

Research output: Contribution to journalArticlepeer-review

Harvard

Kruchinina, MV, Prudnikova, YI, Kurilovich, SA, Gromov, AA, Kruchinin, VN, Atuchin, VV, Naumova, OV, Spesivtsev, EV, Volodin, VA, Peltek, SE, Shuvalov, GV & Generalov, VM 2017, 'Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer', Siberian Journal of Oncology, vol. 16, no. 4, pp. 32-41. https://doi.org/10.21294/1814-4861-2017-16-4-32-41

APA

Kruchinina, M. V., Prudnikova, Y. I., Kurilovich, S. A., Gromov, A. A., Kruchinin, V. N., Atuchin, V. V., Naumova, O. V., Spesivtsev, E. V., Volodin, V. A., Peltek, S. E., Shuvalov, G. V., & Generalov, V. M. (2017). Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer. Siberian Journal of Oncology, 16(4), 32-41. https://doi.org/10.21294/1814-4861-2017-16-4-32-41

Vancouver

Kruchinina MV, Prudnikova YI, Kurilovich SA, Gromov AA, Kruchinin VN, Atuchin VV et al. Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer. Siberian Journal of Oncology. 2017;16(4):32-41. doi: 10.21294/1814-4861-2017-16-4-32-41

Author

Kruchinina, M. V. ; Prudnikova, Ya I. ; Kurilovich, S. A. et al. / Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer. In: Siberian Journal of Oncology. 2017 ; Vol. 16, No. 4. pp. 32-41.

BibTeX

@article{ccde5241a8fa4bce8396e60fd5d3e138,
title = "Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer",
abstract = "Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.",
keywords = "Colorectal cancer, Diagnosis, Ellipsometry, Fluorescence optical spectrometry, Raman spectroscopy, SOI nanowire biosensor, Surface Plasmon resonance, Tumor M2 pyruvate kinase",
author = "Kruchinina, {M. V.} and Prudnikova, {Ya I.} and Kurilovich, {S. A.} and Gromov, {A. A.} and Kruchinin, {V. N.} and Atuchin, {V. V.} and Naumova, {O. V.} and Spesivtsev, {E. V.} and Volodin, {V. A.} and Peltek, {S. E.} and Shuvalov, {G. V.} and Generalov, {V. M.}",
year = "2017",
doi = "10.21294/1814-4861-2017-16-4-32-41",
language = "English",
volume = "16",
pages = "32--41",
journal = "Siberian Journal of Oncology",
issn = "1814-4861",
publisher = "Tomsk National Research Medical Center of the Russian Academy of Sciences",
number = "4",

}

RIS

TY - JOUR

T1 - Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer

AU - Kruchinina, M. V.

AU - Prudnikova, Ya I.

AU - Kurilovich, S. A.

AU - Gromov, A. A.

AU - Kruchinin, V. N.

AU - Atuchin, V. V.

AU - Naumova, O. V.

AU - Spesivtsev, E. V.

AU - Volodin, V. A.

AU - Peltek, S. E.

AU - Shuvalov, G. V.

AU - Generalov, V. M.

PY - 2017

Y1 - 2017

N2 - Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.

AB - Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.

KW - Colorectal cancer

KW - Diagnosis

KW - Ellipsometry

KW - Fluorescence optical spectrometry

KW - Raman spectroscopy

KW - SOI nanowire biosensor

KW - Surface Plasmon resonance

KW - Tumor M2 pyruvate kinase

UR - http://www.scopus.com/inward/record.url?scp=85041611340&partnerID=8YFLogxK

U2 - 10.21294/1814-4861-2017-16-4-32-41

DO - 10.21294/1814-4861-2017-16-4-32-41

M3 - Article

AN - SCOPUS:85041611340

VL - 16

SP - 32

EP - 41

JO - Siberian Journal of Oncology

JF - Siberian Journal of Oncology

SN - 1814-4861

IS - 4

ER -

ID: 9640595