Research output: Contribution to journal › Article › peer-review
Effect of an Extractant on the Composition of the Lipophilic Constituents of the Extracts of Rhodiola rosea L. and on the Extracts’ Activity. / Kukina, T. P.; Shcherbakov, D. N.; Zybkina, A. V. et al.
In: Russian Journal of Bioorganic Chemistry, Vol. 48, No. 7, 01.12.2022, p. 1461-1469.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effect of an Extractant on the Composition of the Lipophilic Constituents of the Extracts of Rhodiola rosea L. and on the Extracts’ Activity
AU - Kukina, T. P.
AU - Shcherbakov, D. N.
AU - Zybkina, A. V.
AU - Elshin, I. A.
AU - Korsakov, V. O.
AU - Salnikova, O. I.
AU - Kolosov, P. V.
AU - Sandag, Ts
AU - Karakai, D. A.
AU - Mordvinova, E. D.
N1 - FUNDING: The study was supported by the Russian Foundation for Basic Research (project nos. 19-34-90076 and 20-54-44016/20).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - The composition of the lipophilic components of the Rhodiola rosea L. plant was studied. Acidic and neutral components were identified by gas chromatography–mass spectrometry. With methyl-tert-butyl ether (MTBE) as an extractant instead of the volatile solvent diethyl ether, lipophilic extract was obtained. Methyl-tert-butyl ether used as an extraction solvent for raw materials has all the advantages of diethyl ether, being free of its disadvantages. It does not form peroxides or produce elevated partial gas pressure due to its higher boiling point. As a result, comparison with databases identified some triterpene, phenolic, and aliphatic acids with chain lengths 12 to 30 carbon atoms, including saturated, unsaturated, and dibasic acids. In addition to the components known from the literature, more than 50 triterpene and aliphatic compounds were detected in the unsaponifiable residue and acidic fractions for the first time. The hexane extract and the product obtained by the stepwise extraction by MTBE after the extraction of low-polarity compounds with hexane were investigated in a similar way. In the case of an extract obtained using MTBE after the extraction of low-polarity components with hexane, extraction of benzoic and cinnamic acids was more efficient compared to the exhaustive extraction of MTBE. These acids are absent from the hexane extract. Ethanol extraction was also carried out to test bioactivity: exhaustive and after hexane and MTBE extraction. Extracts obtained using MTBE and ethanol showed antiviral activity against the Ebola pseudovirus.
AB - The composition of the lipophilic components of the Rhodiola rosea L. plant was studied. Acidic and neutral components were identified by gas chromatography–mass spectrometry. With methyl-tert-butyl ether (MTBE) as an extractant instead of the volatile solvent diethyl ether, lipophilic extract was obtained. Methyl-tert-butyl ether used as an extraction solvent for raw materials has all the advantages of diethyl ether, being free of its disadvantages. It does not form peroxides or produce elevated partial gas pressure due to its higher boiling point. As a result, comparison with databases identified some triterpene, phenolic, and aliphatic acids with chain lengths 12 to 30 carbon atoms, including saturated, unsaturated, and dibasic acids. In addition to the components known from the literature, more than 50 triterpene and aliphatic compounds were detected in the unsaponifiable residue and acidic fractions for the first time. The hexane extract and the product obtained by the stepwise extraction by MTBE after the extraction of low-polarity compounds with hexane were investigated in a similar way. In the case of an extract obtained using MTBE after the extraction of low-polarity components with hexane, extraction of benzoic and cinnamic acids was more efficient compared to the exhaustive extraction of MTBE. These acids are absent from the hexane extract. Ethanol extraction was also carried out to test bioactivity: exhaustive and after hexane and MTBE extraction. Extracts obtained using MTBE and ethanol showed antiviral activity against the Ebola pseudovirus.
KW - Rhodiola rosea L
KW - extractive substances
KW - gold root
KW - methyl-tert-butyl ether
KW - phenolic acids
UR - https://www.scopus.com/inward/record.url?eid=2-s2.0-85145171884&partnerID=40&md5=70614a3eb3f88f0b9b86b7a1aa90541b
UR - https://www.mendeley.com/catalogue/3f9c9301-f590-33fa-ba2e-015b23e507b2/
U2 - 10.1134/S1068162022070147
DO - 10.1134/S1068162022070147
M3 - Article
VL - 48
SP - 1461
EP - 1469
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 7
ER -
ID: 44627857