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Dissection of affinity captured LINE-1 macromolecular complexes. / Taylor, Martin S.; Altukhov, Ilya; Molloy, Kelly R. et al.

In: eLife, Vol. 7, 30094, 08.01.2018.

Research output: Contribution to journal › Article › peer-review

Harvard

Taylor, MS, Altukhov, I, Molloy, KR, Mita, P, Jiang, H, Adney, EM, Wudzinska, A, Badri, S, Ischenko, D, Eng, G, Burns, KH, Fenyö, D, Chait, BT, Alexeev, D, Rout, MP, Boeke, JD & LaCava, J 2018, 'Dissection of affinity captured LINE-1 macromolecular complexes', eLife, vol. 7, 30094. https://doi.org/10.7554/eLife.30094

APA

Taylor, M. S., Altukhov, I., Molloy, K. R., Mita, P., Jiang, H., Adney, E. M., Wudzinska, A., Badri, S., Ischenko, D., Eng, G., Burns, K. H., Fenyö, D., Chait, B. T., Alexeev, D., Rout, M. P., Boeke, J. D., & LaCava, J. (2018). Dissection of affinity captured LINE-1 macromolecular complexes. eLife, 7, [30094]. https://doi.org/10.7554/eLife.30094

Vancouver

Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM et al. Dissection of affinity captured LINE-1 macromolecular complexes. eLife. 2018 Jan 8;7:30094. doi: 10.7554/eLife.30094

Author

Taylor, Martin S. ; Altukhov, Ilya ; Molloy, Kelly R. et al. / Dissection of affinity captured LINE-1 macromolecular complexes. In: eLife. 2018 ; Vol. 7.

BibTeX

@article{ee9b587bab4c419f87e9d864fc9c6c4c,
title = "Dissection of affinity captured LINE-1 macromolecular complexes",
abstract = "Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNAcontaining RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.",
keywords = "Chromatography, Affinity, Endonucleases/analysis, HeLa Cells, Humans, Long Interspersed Nucleotide Elements, Macromolecular Substances/chemistry, Mass Spectrometry, RNA-Directed DNA Polymerase/analysis, RNA/analysis, Ribonucleoproteins/analysis, HOST FACTORS, REVERSE TRANSCRIPTION, L1 RETROTRANSPOSITION, BINDING-PROTEINS, MESSENGER-RNA, REPLICATION FORKS, AMINO-ACIDS, ORF1 PROTEIN, CELL-CULTURE, STRESS GRANULES",
author = "Taylor, {Martin S.} and Ilya Altukhov and Molloy, {Kelly R.} and Paolo Mita and Hua Jiang and Adney, {Emily M.} and Aleksandra Wudzinska and Sana Badri and Dmitry Ischenko and George Eng and Burns, {Kathleen H.} and David Feny{\"o} and Chait, {Brian T.} and Dmitry Alexeev and Rout, {Michael P.} and Boeke, {Jef D.} and John LaCava",
note = "{\textcopyright} 2018, Taylor et al.",
year = "2018",
month = jan,
day = "8",
doi = "10.7554/eLife.30094",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Dissection of affinity captured LINE-1 macromolecular complexes

AU - Taylor, Martin S.

AU - Altukhov, Ilya

AU - Molloy, Kelly R.

AU - Mita, Paolo

AU - Jiang, Hua

AU - Adney, Emily M.

AU - Wudzinska, Aleksandra

AU - Badri, Sana

AU - Ischenko, Dmitry

AU - Eng, George

AU - Burns, Kathleen H.

AU - Fenyö, David

AU - Chait, Brian T.

AU - Alexeev, Dmitry

AU - Rout, Michael P.

AU - Boeke, Jef D.

AU - LaCava, John

N1 - © 2018, Taylor et al.

PY - 2018/1/8

Y1 - 2018/1/8

N2 - Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNAcontaining RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.

AB - Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNAcontaining RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.

KW - Chromatography, Affinity

KW - Endonucleases/analysis

KW - HeLa Cells

KW - Humans

KW - Long Interspersed Nucleotide Elements

KW - Macromolecular Substances/chemistry

KW - Mass Spectrometry

KW - RNA-Directed DNA Polymerase/analysis

KW - RNA/analysis

KW - Ribonucleoproteins/analysis

KW - HOST FACTORS

KW - REVERSE TRANSCRIPTION

KW - L1 RETROTRANSPOSITION

KW - BINDING-PROTEINS

KW - MESSENGER-RNA

KW - REPLICATION FORKS

KW - AMINO-ACIDS

KW - ORF1 PROTEIN

KW - CELL-CULTURE

KW - STRESS GRANULES

UR - http://www.scopus.com/inward/record.url?scp=85043495559&partnerID=8YFLogxK

U2 - 10.7554/eLife.30094

DO - 10.7554/eLife.30094

M3 - Article

C2 - 29309035

AN - SCOPUS:85043495559

VL - 7

JO - eLife

JF - eLife

SN - 2050-084X

M1 - 30094

ER -

ID: 12080934