TY - JOUR

T1 - Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

AU - Kochetov, Alex V.

AU - Glagoleva, Anastasiya Y.

AU - Strygina, Kseniya V.

AU - Khlestkina, Elena K.

AU - Gerasimova, Sophia V.

AU - Ibragimova, Salmaz M.

AU - Shatskaya, Natalja V.

AU - Vasilyev, Gennady V.

AU - Afonnikov, Dmitry A.

AU - Shmakov, Nikolay A.

AU - Antonova, Olga Y.

AU - Gavrilenko, Tatyana A.

AU - Alpatyeva, Natalia V.

AU - Khiutti, Alexander

AU - Afanasenko, Olga S.

PY - 2017/12/28

Y1 - 2017/12/28

N2 - Background: The characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming. Results: Comparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode. Conclusion: Combination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.

AB - Background: The characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming. Results: Comparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode. Conclusion: Combination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.

KW - Globodera rostochiensis

KW - NBS-LRR genes

KW - Resistance

KW - Solanum phureja

KW - Gene Expression Regulation, Plant/genetics

KW - Genes, Plant/genetics

KW - Animals

KW - Tylenchoidea/physiology

KW - Solanum/genetics

KW - Disease Resistance/genetics

KW - Transcriptome/genetics

KW - Plant Roots/metabolism

KW - NICOTIANA-BENTHAMIANA

KW - POTATO

KW - HIGH-RESOLUTION MAP

KW - NEMATODE

KW - CONFERRING RESISTANCE

KW - ENHANCES DISEASE RESISTANCE

KW - RNA-SEQ

KW - BROAD-SPECTRUM RESISTANCE

KW - CANDIDATE GENE

KW - HYPERSENSITIVE RESPONSE

UR - http://www.scopus.com/inward/record.url?scp=85039730790&partnerID=8YFLogxK

U2 - 10.1186/s12870-017-1193-1

DO - 10.1186/s12870-017-1193-1

M3 - Article

C2 - 29297325

AN - SCOPUS:85039730790

VL - 17

SP - 251

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

IS - Suppl 2

M1 - 251

ER -