Determining blood platelet morphology modelled by a superellipsoid from the solution of the inverse light-scattering problem

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Determining blood platelet morphology modelled by a superellipsoid from the solution of the inverse light-scattering problem. / Litvinenko, Alena L.; Nekrasov, Vyacheslav M.; Gilev, Konstantin V. et al.

In: Optics and Laser Technology, Vol. 176, 110881, 2024.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{cbd942fe7b7c4d77a9519bc2fb6f75a9,

title = "Determining blood platelet morphology modelled by a superellipsoid from the solution of the inverse light-scattering problem",

abstract = "In this work, we propose a new optical model to describe the morphology of a platelet in the form of a special axisymmetric superellipsoid. This model is more general than the widely used classical oblate spheroid model of the platelet and includes the oblate spheroid as a special case. The inverse light scattering (ILS) problem within the developed optical model was solved using the light scattering profile (LSP) of individual platelets obtained by the scanning flow cytometer. Solving the ILS problem we used a hybrid method that combines the DIRECT algorithm of global optimization with the linear interpolation between the nodes of the pre-calculated regular database of superellipsoid LSPs. The statistical F-test shows that the new optical model is better than the conventional oblate spheroid model in more than 60 percent of cases. This made it possible to obtain the refined shape index distributions for platelets consisting of three fractions, namely resting, partially and fully activated platelets.",

keywords = "Inverse problem, Light scattering, Oblate spheroid, Platelets, Scanning flow cytometry, Superellipsoid optical model",

author = "Litvinenko, {Alena L.} and Nekrasov, {Vyacheslav M.} and Gilev, {Konstantin V.} and Alexandrov, {Evgeniy A.} and Strokotov, {Dmitry I.} and Maltsev, {Valeri P.} and Karpenko, {Andrey A.} and Yastrebova, {Ekaterina S.}",

note = "This work was supported by the Russian Science Foundation (grant No. 23-25-00347).",

year = "2024",

doi = "10.1016/j.optlastec.2024.110881",

language = "English",

volume = "176",

journal = "Optics and Laser Technology",

issn = "0030-3992",

publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Determining blood platelet morphology modelled by a superellipsoid from the solution of the inverse light-scattering problem

AU - Litvinenko, Alena L.

AU - Nekrasov, Vyacheslav M.

AU - Gilev, Konstantin V.

AU - Alexandrov, Evgeniy A.

AU - Strokotov, Dmitry I.

AU - Maltsev, Valeri P.

AU - Karpenko, Andrey A.

AU - Yastrebova, Ekaterina S.

N1 - This work was supported by the Russian Science Foundation (grant No. 23-25-00347).

PY - 2024

Y1 - 2024

N2 - In this work, we propose a new optical model to describe the morphology of a platelet in the form of a special axisymmetric superellipsoid. This model is more general than the widely used classical oblate spheroid model of the platelet and includes the oblate spheroid as a special case. The inverse light scattering (ILS) problem within the developed optical model was solved using the light scattering profile (LSP) of individual platelets obtained by the scanning flow cytometer. Solving the ILS problem we used a hybrid method that combines the DIRECT algorithm of global optimization with the linear interpolation between the nodes of the pre-calculated regular database of superellipsoid LSPs. The statistical F-test shows that the new optical model is better than the conventional oblate spheroid model in more than 60 percent of cases. This made it possible to obtain the refined shape index distributions for platelets consisting of three fractions, namely resting, partially and fully activated platelets.

AB - In this work, we propose a new optical model to describe the morphology of a platelet in the form of a special axisymmetric superellipsoid. This model is more general than the widely used classical oblate spheroid model of the platelet and includes the oblate spheroid as a special case. The inverse light scattering (ILS) problem within the developed optical model was solved using the light scattering profile (LSP) of individual platelets obtained by the scanning flow cytometer. Solving the ILS problem we used a hybrid method that combines the DIRECT algorithm of global optimization with the linear interpolation between the nodes of the pre-calculated regular database of superellipsoid LSPs. The statistical F-test shows that the new optical model is better than the conventional oblate spheroid model in more than 60 percent of cases. This made it possible to obtain the refined shape index distributions for platelets consisting of three fractions, namely resting, partially and fully activated platelets.

KW - Inverse problem

KW - Light scattering

KW - Oblate spheroid

KW - Platelets

KW - Scanning flow cytometry

KW - Superellipsoid optical model

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85188887447&origin=inward&txGid=0becf9705bfd9dc8ef5663317020d9b7

UR - https://www.mendeley.com/catalogue/68abc746-ac86-3965-9b85-2242b584660c/

U2 - 10.1016/j.optlastec.2024.110881

DO - 10.1016/j.optlastec.2024.110881

M3 - Article

VL - 176

JO - Optics and Laser Technology

JF - Optics and Laser Technology

SN - 0030-3992

M1 - 110881

ER -

ID: 60816044