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Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy. / Dementyeva, E. V.; Medvedev, S. P.; Kovalenko, V. R. et al.

In: Biochemistry (Moscow), Vol. 84, No. 3, 01.03.2019, p. 291-298.

Research output: Contribution to journal › Article › peer-review

Harvard

Dementyeva, EV, Medvedev, SP, Kovalenko, VR, Vyatkin, YV, Kretov, EI, Slotvitsky, MM, Shtokalo, DN, Pokushalov, EA & Zakian, SM 2019, 'Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy', Biochemistry (Moscow), vol. 84, no. 3, pp. 291-298. https://doi.org/10.1134/S0006297919030118

APA

Dementyeva, E. V., Medvedev, S. P., Kovalenko, V. R., Vyatkin, Y. V., Kretov, E. I., Slotvitsky, M. M., Shtokalo, D. N., Pokushalov, E. A., & Zakian, S. M. (2019). Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy. Biochemistry (Moscow), 84(3), 291-298. https://doi.org/10.1134/S0006297919030118

Vancouver

Dementyeva EV, Medvedev SP, Kovalenko VR, Vyatkin YV, Kretov EI, Slotvitsky MM et al. Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy. Biochemistry (Moscow). 2019 Mar 1;84(3):291-298. doi: 10.1134/S0006297919030118

Author

Dementyeva, E. V. ; Medvedev, S. P. ; Kovalenko, V. R. et al. / Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy. In: Biochemistry (Moscow). 2019 ; Vol. 84, No. 3. pp. 291-298.

BibTeX

@article{cb3634115ddf492a9a179ba967f70329,
title = "Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy",
abstract = "Generation of patient-specific induced pluripotent stem cells (iPSCs) and their subsequent differentiation into cardiomyocytes opened new opportunities for studying pathogenesis of inherited cardiovascular diseases. One of these diseases is hypertrophic cardiomyopathy (HCM) for which no efficient therapy methods have been developed so far. In this study, the approach based on patient-specific iPSCs was applied to create a model of the disease. Genetic analysis of a hypertrophic cardiomyopathy patient revealed R326Q mutation in the MYBPC3 gene. iPSCs of the patient were generated and characterized. The cells were differentiated into cardiomyocytes together with the control iPSCs from a healthy donor. The patient{\textquoteright}s iPSC-derived cardiomyocytes exhibited early HCM features, such as abnormal calcium handling and increased intracellular calcium concentration. Therefore, cardiomyocytes obtained by directed differentiation of iPSCs from the HCM patient can be used as a model system to study HCM pathogenesis.",
keywords = "cardiomyocytes, human disease models, hypertrophic cardiomyopathy, induced pluripotent stem cells",
author = "Dementyeva, {E. V.} and Medvedev, {S. P.} and Kovalenko, {V. R.} and Vyatkin, {Yu V.} and Kretov, {E. I.} and Slotvitsky, {M. M.} and Shtokalo, {D. N.} and Pokushalov, {E. A.} and Zakian, {S. M.}",
note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Ltd.",
year = "2019",
month = mar,
day = "1",
doi = "10.1134/S0006297919030118",
language = "English",
volume = "84",
pages = "291--298",
journal = "Biochemistry (Moscow)",
issn = "0006-2979",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "3",

}

RIS

TY - JOUR

T1 - Applying Patient-Specific Induced Pluripotent Stem Cells to Create a Model of Hypertrophic Cardiomyopathy

AU - Dementyeva, E. V.

AU - Medvedev, S. P.

AU - Kovalenko, V. R.

AU - Vyatkin, Yu V.

AU - Kretov, E. I.

AU - Slotvitsky, M. M.

AU - Shtokalo, D. N.

AU - Pokushalov, E. A.

AU - Zakian, S. M.

N1 - Publisher Copyright: © 2019, Pleiades Publishing, Ltd.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Generation of patient-specific induced pluripotent stem cells (iPSCs) and their subsequent differentiation into cardiomyocytes opened new opportunities for studying pathogenesis of inherited cardiovascular diseases. One of these diseases is hypertrophic cardiomyopathy (HCM) for which no efficient therapy methods have been developed so far. In this study, the approach based on patient-specific iPSCs was applied to create a model of the disease. Genetic analysis of a hypertrophic cardiomyopathy patient revealed R326Q mutation in the MYBPC3 gene. iPSCs of the patient were generated and characterized. The cells were differentiated into cardiomyocytes together with the control iPSCs from a healthy donor. The patient’s iPSC-derived cardiomyocytes exhibited early HCM features, such as abnormal calcium handling and increased intracellular calcium concentration. Therefore, cardiomyocytes obtained by directed differentiation of iPSCs from the HCM patient can be used as a model system to study HCM pathogenesis.

AB - Generation of patient-specific induced pluripotent stem cells (iPSCs) and their subsequent differentiation into cardiomyocytes opened new opportunities for studying pathogenesis of inherited cardiovascular diseases. One of these diseases is hypertrophic cardiomyopathy (HCM) for which no efficient therapy methods have been developed so far. In this study, the approach based on patient-specific iPSCs was applied to create a model of the disease. Genetic analysis of a hypertrophic cardiomyopathy patient revealed R326Q mutation in the MYBPC3 gene. iPSCs of the patient were generated and characterized. The cells were differentiated into cardiomyocytes together with the control iPSCs from a healthy donor. The patient’s iPSC-derived cardiomyocytes exhibited early HCM features, such as abnormal calcium handling and increased intracellular calcium concentration. Therefore, cardiomyocytes obtained by directed differentiation of iPSCs from the HCM patient can be used as a model system to study HCM pathogenesis.

KW - cardiomyocytes

KW - human disease models

KW - hypertrophic cardiomyopathy

KW - induced pluripotent stem cells

UR - http://www.scopus.com/inward/record.url?scp=85063502415&partnerID=8YFLogxK

U2 - 10.1134/S0006297919030118

DO - 10.1134/S0006297919030118

M3 - Article

C2 - 31221067

AN - SCOPUS:85063502415

VL - 84

SP - 291

EP - 298

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 3

ER -

ID: 19028846