Research output: Contribution to journal › Article › peer-review
3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox. / Lavrekha, Viktoriya V.; Pasternak, Taras; Palme, Klaus et al.
In: Methods in molecular biology (Clifton, N.J.), Vol. 2094, 01.01.2020, p. 119-125.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox
AU - Lavrekha, Viktoriya V.
AU - Pasternak, Taras
AU - Palme, Klaus
AU - Mironova, Victoria V.
N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
AB - The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
KW - Arabidopsis
KW - Cell cycle
KW - Cortex
KW - Endodermis
KW - Epidermis
KW - Image analysis
KW - Pericycle
KW - Proliferation
KW - Protophloem
KW - Protoxylem
KW - RAM
UR - http://www.scopus.com/inward/record.url?scp=85076108828&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-0183-9_13
DO - 10.1007/978-1-0716-0183-9_13
M3 - Article
C2 - 31797297
AN - SCOPUS:85076108828
VL - 2094
SP - 119
EP - 125
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1940-6029
ER -
ID: 22576706