Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype. / Litunenko, Daria N; Moskalensky, Alexander E.
в: Journal of Biophotonics, Том 17, № 1, e202300234, 01.2024.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype
AU - Litunenko, Daria N
AU - Moskalensky, Alexander E
N1 - The study was supported by the Ministr y of Science and Higher Education of the Russ ia n Federation (project FSUS-2020-0039). This article is protected by copyright. All rights reserved. © 2023 Wiley-VCH GmbH.
PY - 2024/1
Y1 - 2024/1
N2 - Cell cultures are widely used in scientific research, biomedicine and industry. When culturing, it is important to maintain certain conditions, including the concentration of cells. Monitoring of the culture growth and cell counting is an urgent task for the optimization of technological processes. Most existing methods require sampling from a culture flask. This procedure is time-consuming and associated with the risks of contamination. We present a device able to monitor the growth of cells number in a suspension non-invasively. The device uses a laser beam that pass through the culture flask and measures the intensity of scattered light as a function of coordinate along the beam. This optical scheme allows one to obtain accurate results for both high- and low-scattering samples. We constructed the wireless portable prototype for monitoring of cell culture growth directly in the incubator and demonstrated the applicability of the device for Jurkat cells and e.coli bacteria. This article is protected by copyright. All rights reserved.
AB - Cell cultures are widely used in scientific research, biomedicine and industry. When culturing, it is important to maintain certain conditions, including the concentration of cells. Monitoring of the culture growth and cell counting is an urgent task for the optimization of technological processes. Most existing methods require sampling from a culture flask. This procedure is time-consuming and associated with the risks of contamination. We present a device able to monitor the growth of cells number in a suspension non-invasively. The device uses a laser beam that pass through the culture flask and measures the intensity of scattered light as a function of coordinate along the beam. This optical scheme allows one to obtain accurate results for both high- and low-scattering samples. We constructed the wireless portable prototype for monitoring of cell culture growth directly in the incubator and demonstrated the applicability of the device for Jurkat cells and e.coli bacteria. This article is protected by copyright. All rights reserved.
KW - cell culture
KW - light scattering
KW - nephelometry
KW - optical devices
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85174241873&origin=inward&txGid=33f8472ac9b981b8941d7bc5a8c41aed
UR - https://www.mendeley.com/catalogue/655d5e15-41a8-3921-9b55-e42f1a802e26/
U2 - 10.1002/jbio.202300234
DO - 10.1002/jbio.202300234
M3 - Article
C2 - 37795552
VL - 17
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 1
M1 - e202300234
ER -
ID: 55685771