TY - JOUR

T1 - Vasopressin-Dependent Water Permeability Regulation in Collecting Duct Principal Cells of Brattleboro Rats

T2 - the Role of cAMP

AU - Baturina, G. S.

AU - Katkova, L. E.

AU - Solenov, E. I.

N1 - Baturina, G. S. Vasopressin-Dependent Water Permeability Regulation in Collecting Duct Principal Cells of Brattleboro Rats: the Role of cAMP / G. S. Baturina, L. E. Katkova, E. I. Solenov // Journal of Evolutionary Biochemistry and Physiology. – 2025. – Vol. 61, No. 6. – P. 1748-1756. – DOI 10.1134/S0022093025060079. – EDN WWTKKH. The work was carried out within the governmental assignment to the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (FWNR-2022-0019СО). No additional grants to conduct or supervise this particular research were obtained.

PY - 2025/12/19

Y1 - 2025/12/19

N2 - Vasopressin (antidiuretic hormone, ADH) plays an important role in the regulation of water–salt balance in mammals. Brattleboro rats lack endogenous ADH due to a mutation in the ADH-neurophysin gene, leading to a recessive form of central diabetes insipidus. This work investigated the role of cAMP in ADH-dependent regulation of water permeability of the apical and basolateral membranes in outer medullary collecting duct (OMCD) principal cells in Brattleboro rats. Data obtained from water-loaded Wistar rats were used as a control. The osmotic water permeability coefficient (P f) of principal cells in isolated OMCD was assessed using a method based on calcein fluorescence quenching by cytoplasmic proteins. The content of aquaporins AQP2 and AQP3 in the membrane fraction of outer medullary cells was evaluated by Western blotting. Both the vasopressin V2 receptor agonist (DDAVP, 10–8 M) and the cell-permeable cAMP analog (DB-cAMP, 10–5 M) significantly increased the water permeability of both the apical and basolateral membranes of OMCD principal cells in both rat strains after a 25-min exposure, with the cAMP analog having a more pronounced effect compared to DDAVP. We have previously shown that short-term exposure to DDAVP increased AQP2 content in the membrane fraction of Wistar rats and AQP2-3 content in Brattleboro rats. In this study, Western blotting demonstrated that DB-cAMP increased the content of AQP2, but not AQP3, in the membrane fraction of the renal outer medulla in both Wistar and Brattleboro rats. It can be assumed that in Brattleboro rats, AQP3-mediated water transport across the basolateral membrane of OMCD principal cells is regulated by ADH not only through a cAMP-dependent pathway but also with the involvement of other, including calcium-related, secondary messenger systems.

AB - Vasopressin (antidiuretic hormone, ADH) plays an important role in the regulation of water–salt balance in mammals. Brattleboro rats lack endogenous ADH due to a mutation in the ADH-neurophysin gene, leading to a recessive form of central diabetes insipidus. This work investigated the role of cAMP in ADH-dependent regulation of water permeability of the apical and basolateral membranes in outer medullary collecting duct (OMCD) principal cells in Brattleboro rats. Data obtained from water-loaded Wistar rats were used as a control. The osmotic water permeability coefficient (P f) of principal cells in isolated OMCD was assessed using a method based on calcein fluorescence quenching by cytoplasmic proteins. The content of aquaporins AQP2 and AQP3 in the membrane fraction of outer medullary cells was evaluated by Western blotting. Both the vasopressin V2 receptor agonist (DDAVP, 10–8 M) and the cell-permeable cAMP analog (DB-cAMP, 10–5 M) significantly increased the water permeability of both the apical and basolateral membranes of OMCD principal cells in both rat strains after a 25-min exposure, with the cAMP analog having a more pronounced effect compared to DDAVP. We have previously shown that short-term exposure to DDAVP increased AQP2 content in the membrane fraction of Wistar rats and AQP2-3 content in Brattleboro rats. In this study, Western blotting demonstrated that DB-cAMP increased the content of AQP2, but not AQP3, in the membrane fraction of the renal outer medulla in both Wistar and Brattleboro rats. It can be assumed that in Brattleboro rats, AQP3-mediated water transport across the basolateral membrane of OMCD principal cells is regulated by ADH not only through a cAMP-dependent pathway but also with the involvement of other, including calcium-related, secondary messenger systems.

KW - вазопрессин

KW - аквапорины

KW - крысы Браттлборо

KW - цАМФ

KW - регуляция проницаемости воды

KW - несахарный диабет

UR - https://www.mendeley.com/catalogue/b2b61187-28ce-3e44-9b8e-d3a18b885a03/

UR - https://www.elibrary.ru/item.asp?id=87364849

U2 - 10.1134/s0022093025060079

DO - 10.1134/s0022093025060079

M3 - Article

VL - 61

SP - 1748

EP - 1756

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 6

ER -