TY - JOUR

T1 - Vapor-Phase-Deposited Ag/Ir and Ag/Au Film Heterostructures for Implant Materials: Cytotoxic, Antibacterial and Histological Studie

T2 - s

AU - Sergeevichev, David S

AU - Dorovskikh, Svetlana I

AU - Vikulova, Evgeniia S

AU - Chepeleva, Elena V

AU - Vasiliyeva, Maria B

AU - Koretskaya, Tatiana P

AU - Fedorenko, Anastasiya D

AU - Nasimov, Dmitriy A

AU - Guselnikova, Tatiana Y

AU - Popovetsky, Pavel S

AU - Morozova, Natalya B

AU - Basova, Tamara V

N1 - This research was funded by the Russian Science Foundation for the funding project N 20-15-00222-P. The authors thank the Ministry of Education and Science of the Russian Federation (projects 121031700314-5 and 121031700313-8) for access to the literature search databases and the equipment of the Collaborative Use Center to determine the film composition and morphology.

PY - 2024/1

Y1 - 2024/1

N2 - Using gas-phase deposition (Physical Vapor Deposition (PVD) and Metal Organic Chemical Vapor Deposition (MOCVD)) methods, modern implant samples (Ti alloy and CFR-PEEK polymer, 30% carbon fiber) were functionalized with film heterostructures consisting of an iridium or gold sublayer, on the surface of which an antibacterial component (silver) was deposited: Ag/Ir(Au)/Ti(CFR-PEEK). The biocidal effect of the heterostructures was investigated, the effect of the surface relief of the carrier and the metal sublayer on antibacterial activity was established, and the dynamics of silver dissolution was evaluated. It has been shown that the activity of Ag/Ir heterostructures was due to high Ag+ release rates, which led to rapid (2-4 h) inhibition of P. aeruginosa growth. In the case of Ag/Au type heterostructures, the inhibition of the growth of P. aeruginosa and S. aureus occurred more slowly (from 6 h), and the antibacterial activity appeared to be due to the contribution of two agents (Ag+ and Au+ ions). It was found, according to the in vitro cytotoxicity study, that heterostructures did not exhibit toxic effects (cell viability > 95-98%). An in vivo biocompatibility assessment based on the results of a morphohistological study showed that after implantation for a period of 30 days, the samples were characterized by the presence of a thin fibrous capsule without volume thickening and signs of inflammation.

AB - Using gas-phase deposition (Physical Vapor Deposition (PVD) and Metal Organic Chemical Vapor Deposition (MOCVD)) methods, modern implant samples (Ti alloy and CFR-PEEK polymer, 30% carbon fiber) were functionalized with film heterostructures consisting of an iridium or gold sublayer, on the surface of which an antibacterial component (silver) was deposited: Ag/Ir(Au)/Ti(CFR-PEEK). The biocidal effect of the heterostructures was investigated, the effect of the surface relief of the carrier and the metal sublayer on antibacterial activity was established, and the dynamics of silver dissolution was evaluated. It has been shown that the activity of Ag/Ir heterostructures was due to high Ag+ release rates, which led to rapid (2-4 h) inhibition of P. aeruginosa growth. In the case of Ag/Au type heterostructures, the inhibition of the growth of P. aeruginosa and S. aureus occurred more slowly (from 6 h), and the antibacterial activity appeared to be due to the contribution of two agents (Ag+ and Au+ ions). It was found, according to the in vitro cytotoxicity study, that heterostructures did not exhibit toxic effects (cell viability > 95-98%). An in vivo biocompatibility assessment based on the results of a morphohistological study showed that after implantation for a period of 30 days, the samples were characterized by the presence of a thin fibrous capsule without volume thickening and signs of inflammation.

KW - Silver/pharmacology

KW - Staphylococcus aureus

KW - Polymers/pharmacology

KW - Anti-Bacterial Agents/pharmacology

KW - Antineoplastic Agents

KW - Gases

KW - Benzophenones

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85183232304&origin=inward&txGid=321d4259f237f8f01d869c348b949888

U2 - 10.3390/ijms25021100

DO - 10.3390/ijms25021100

M3 - Article

C2 - 38256173

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 2

M1 - 1100

ER -