TY - JOUR

T1 - Validation of Heterophase RNA Analysis with the Use of a Silicon-on-Insulator Biosensor

AU - Dmitrienko, E. V.

AU - Poryvaeva, A. V.

AU - Naumova, O. V.

AU - Fomin, B. I.

AU - Kupryushkin, M. S.

AU - Pyshnaya, I. A.

AU - Pyshnyi, D. V.

N1 - Funding Information: This study was supported by the Ministry of Science and Higher Education of the Russian Federation (state registration no. AAAA-A17-117020210021-7) and the Russian Science Foundation (grant no. 18-14-00357). Funding Information: We are grateful to the specialists of the Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences M. I. Meshchaninova for the analysis of fluorescently marked RNA sequences 1?3 and D. V. Semenov for the granted sequences of diagnostically significant RNAs. Publisher Copyright: © 2021, Allerton Press, Inc.

PY - 2021/1

Y1 - 2021/1

N2 - The analysis of nucleic acids remains to be a topical trend in the development of medical diagnostics. Contemporary ultrasensitive diagnostic systems provide the conversion of a specific interaction into a hardwarely-detectable signal in the course of analysis. An example of such diagnostic devices is silicon-on-insulator (SOI) biosensors of field-effect transistors (FET). In this study, glass surfaces are used (as a Si/SiO2 surface for a SOI biosensor) for the optimization and validation of all analysis stages. The efficient immobilization of electroneutral analogs of oligonucleides onto a Si/SiO2surface after the activation of Si-OH groups with 3-glycidoxypropyltrimethoxysilane or carbonyldiimidazole is demonstrated. In the presence of salt at a low concentration or with no salt, the possibility of detecting an RNA target on model glass surfaces in the parallel analysis regime is shown. The regeneration of the Si/SiO2 surface of sensors for repeated analysis and the stability of sensors under long-term storage are also demonstrated.

AB - The analysis of nucleic acids remains to be a topical trend in the development of medical diagnostics. Contemporary ultrasensitive diagnostic systems provide the conversion of a specific interaction into a hardwarely-detectable signal in the course of analysis. An example of such diagnostic devices is silicon-on-insulator (SOI) biosensors of field-effect transistors (FET). In this study, glass surfaces are used (as a Si/SiO2 surface for a SOI biosensor) for the optimization and validation of all analysis stages. The efficient immobilization of electroneutral analogs of oligonucleides onto a Si/SiO2surface after the activation of Si-OH groups with 3-glycidoxypropyltrimethoxysilane or carbonyldiimidazole is demonstrated. In the presence of salt at a low concentration or with no salt, the possibility of detecting an RNA target on model glass surfaces in the parallel analysis regime is shown. The regeneration of the Si/SiO2 surface of sensors for repeated analysis and the stability of sensors under long-term storage are also demonstrated.

KW - electroneutral phosphoryl guanidine oligonucleotides

KW - heterophase analysis

KW - hybridization

KW - Si/SiO surfaces

KW - SOI biosensor

UR - http://www.scopus.com/inward/record.url?scp=85109932896&partnerID=8YFLogxK

U2 - 10.3103/S8756699021010039

DO - 10.3103/S8756699021010039

M3 - Article

AN - SCOPUS:85109932896

VL - 57

SP - 44

EP - 50

JO - Optoelectronics, Instrumentation and Data Processing

JF - Optoelectronics, Instrumentation and Data Processing

SN - 8756-6990

IS - 1

ER -