TY - JOUR

T1 - Two-Pulse Electron Spin Echo of Biradicals: Dependence on Pulse Turning Angles

AU - Syryamina, Victoria N.

AU - Rogozhnikova, Olga Yu

AU - Tormyshev, Victor M.

AU - Dzuba, Sergei A.

N1 - V.N.S. and S.A.D. acknowledge the core funding from the Russian Federal Ministry of Science and Higher Education (FWGF-2021-0003), O.Yu.R. and V.M.T. acknowledge the core funding from the Russian Federal Ministry of Science and Higher Education (FWUE-2022-0012).

PY - 2024/1/1

Y1 - 2024/1/1

N2 - Double spin labeling is often used to study the conformations of biomolecules using pulsed dipolar EPR spectroscopy (PDS). For spin labels based on triarylmethyl (TAM) radicals, there is a data processing problem due to the strong dipole–dipole coupling of the two spins due to the narrow EPR line. As recently shown, these problems can be overcome in a simple PDS approach based on two-pulse electron spin echo spectroscopy (2p ESE). Although the theory underlying this approach was developed by Salikhov and Zhidomirov many years ago, all of its predictions, however, have yet to be tested. Here we obtain additional arguments in favor of the applicability of this theory by investigating the dependence of the echo signal on the pulse turning angles. In addition, we show how the results obtained can be useful for data processing in PDS methods.

AB - Double spin labeling is often used to study the conformations of biomolecules using pulsed dipolar EPR spectroscopy (PDS). For spin labels based on triarylmethyl (TAM) radicals, there is a data processing problem due to the strong dipole–dipole coupling of the two spins due to the narrow EPR line. As recently shown, these problems can be overcome in a simple PDS approach based on two-pulse electron spin echo spectroscopy (2p ESE). Although the theory underlying this approach was developed by Salikhov and Zhidomirov many years ago, all of its predictions, however, have yet to be tested. Here we obtain additional arguments in favor of the applicability of this theory by investigating the dependence of the echo signal on the pulse turning angles. In addition, we show how the results obtained can be useful for data processing in PDS methods.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85212102086&origin=inward&txGid=01db6bf3a5e74908a7789dfe68a0760d

UR - https://www.mendeley.com/catalogue/0b3878a3-fb52-362a-9c0d-17d95841e5b6/

U2 - 10.1007/s00723-024-01740-1

DO - 10.1007/s00723-024-01740-1

M3 - Article

JO - Applied Magnetic Resonance

JF - Applied Magnetic Resonance

SN - 0937-9347

M1 - 107363

ER -