Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Toward gene therapy of hypertension : Experimental study on hypertensive ISIAH rats. / Repkova, M. N.; Levina, A. S.; Seryapina, A. A. и др.
в: Biochemistry (Moscow), Том 82, № 4, 01.04.2017, стр. 454-457.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Toward gene therapy of hypertension
T2 - Experimental study on hypertensive ISIAH rats
AU - Repkova, M. N.
AU - Levina, A. S.
AU - Seryapina, A. A.
AU - Shikina, N. V.
AU - Bessudnova, E. V.
AU - Zarytova, V. F.
AU - Markel, A. L.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - TiO2-based nanocomposites were prepared to deliver oligonucleotides into cells. The nanocomposites were designed by the immobilization of polylysine-containing oligonucleotides on TiO2-nanoparticles (TiO2·PL-DNA). We showed for the first time the possibility of using the proposed nanocomposites for treatment of hypertensive disease by introducing them into hypertensive ISIAH rats developed as a model of stress-sensitive arterial hypertension. The mRNA of the gene encoding angiotensin I-converting enzyme (ACE1) involved in the synthesis of angiotensin II was chosen as a target. Administration (intraperitoneal injection and inhalation) of the nanocomposite showed a significant (by 20-30 mm Hg) decrease in systolic blood pressure when the nanocomposite contained the ACE1 gene-targeted oligonucleotide. When using the oligonucleotide with a random sequence, no effect was observed. Further development and improvement of the inhalation nanocomposite drug delivery to systemic hypertensive disease treatment promises new possibilities for clinical practice.
AB - TiO2-based nanocomposites were prepared to deliver oligonucleotides into cells. The nanocomposites were designed by the immobilization of polylysine-containing oligonucleotides on TiO2-nanoparticles (TiO2·PL-DNA). We showed for the first time the possibility of using the proposed nanocomposites for treatment of hypertensive disease by introducing them into hypertensive ISIAH rats developed as a model of stress-sensitive arterial hypertension. The mRNA of the gene encoding angiotensin I-converting enzyme (ACE1) involved in the synthesis of angiotensin II was chosen as a target. Administration (intraperitoneal injection and inhalation) of the nanocomposite showed a significant (by 20-30 mm Hg) decrease in systolic blood pressure when the nanocomposite contained the ACE1 gene-targeted oligonucleotide. When using the oligonucleotide with a random sequence, no effect was observed. Further development and improvement of the inhalation nanocomposite drug delivery to systemic hypertensive disease treatment promises new possibilities for clinical practice.
KW - antisense oligonucleotides
KW - hypertension
KW - ISIAH rats
KW - nanocomposites
KW - NANOPARTICLES
KW - NANOCOMPOSITES
KW - INFLUENZA-A VIRUS
KW - ANTISENSE
KW - DISEASE
KW - STRESS
KW - BLOOD-PRESSURE
KW - DELIVERY
UR - http://www.scopus.com/inward/record.url?scp=85017514264&partnerID=8YFLogxK
U2 - 10.1134/S000629791704006X
DO - 10.1134/S000629791704006X
M3 - Article
C2 - 28371602
AN - SCOPUS:85017514264
VL - 82
SP - 454
EP - 457
JO - Biochemistry (Moscow)
JF - Biochemistry (Moscow)
SN - 0006-2979
IS - 4
ER -
ID: 8673295