TY - JOUR

T1 - Toward a Comprehensive Overview of Cytogenomics in the Galliformes: A Focus on Western Capercaillie and Hazel Grouse Karyotypes

AU - Proskuryakova, Anastasia A.

AU - Ivanova, Ekaterina S.

AU - Galkina, Svetlana

AU - Beklemisheva, Violetta R.

AU - Kulak, Maria M.

AU - Serdyukova, Natalia A.

AU - Yudkin, Vladimir A.

AU - O'Connor, Rebecca

AU - Griffin, Darren K.

AU - Graphodatsky, Alexander S.

N1 - This research was supported by Russian Science Foundation Grant No. 23-74-01016 (to Anastasia A. Proskuryakova).

PY - 2025/3/11

Y1 - 2025/3/11

N2 - Introduction: The Phasianidae family belongs to Galliformes, which is basal to other Neognathae. Despite the availability of chromosome-level genome assemblies for many Phasianidae species, the karyotypes for some species remain poorly investigated. Methods: In this study, we described karyotypes using classical, differential, and molecular cytogenetic (BAC-FISH) methods. To compare chromosome-level genomes of 10 Galliformes species dot-plot analysis was performed. Results: We provide the first comprehensive description of the karyotype of two Tetraonini species: the western capercaillie (Tetrao urogallus, 2n = 78) and the hazel grouse (Tetrastes bonasia, 2n = 80). We mapped chicken BAC clones (CHORI-261) with known coordinates to the chromosomes of the western capercaillie and Japanese quail (Coturnix japonica, 2n = 78) to anchor physical chromosomes to chromosome-level assemblies. Finally, we performed dot-plot comparisons of ten available chromosome-level genome assemblies to identify interand intrachromosomal rearrangements in Galliformes. Conclusion: We show that the centromeric fusion of orthologs of GGA6 and GGA8 is shared by all analyzed species in the tetraonid clade: T. urogallus, T. bonasia, and Lagopus muta. We identified linage-specific intrachromosomal rearrangements on chromosomes orthologs to chicken Z (Phasianinae and Tetraoninae), 7 and 12 (Phasianinae and Tetraoninae), 5 and 13 (Perdicinae), 22 (Alectoris). Our study shows that analysis of the genomes of several closely related species allows us to identify chromosomal rearrangements characteristic of individual evolutionary lines.

AB - Introduction: The Phasianidae family belongs to Galliformes, which is basal to other Neognathae. Despite the availability of chromosome-level genome assemblies for many Phasianidae species, the karyotypes for some species remain poorly investigated. Methods: In this study, we described karyotypes using classical, differential, and molecular cytogenetic (BAC-FISH) methods. To compare chromosome-level genomes of 10 Galliformes species dot-plot analysis was performed. Results: We provide the first comprehensive description of the karyotype of two Tetraonini species: the western capercaillie (Tetrao urogallus, 2n = 78) and the hazel grouse (Tetrastes bonasia, 2n = 80). We mapped chicken BAC clones (CHORI-261) with known coordinates to the chromosomes of the western capercaillie and Japanese quail (Coturnix japonica, 2n = 78) to anchor physical chromosomes to chromosome-level assemblies. Finally, we performed dot-plot comparisons of ten available chromosome-level genome assemblies to identify interand intrachromosomal rearrangements in Galliformes. Conclusion: We show that the centromeric fusion of orthologs of GGA6 and GGA8 is shared by all analyzed species in the tetraonid clade: T. urogallus, T. bonasia, and Lagopus muta. We identified linage-specific intrachromosomal rearrangements on chromosomes orthologs to chicken Z (Phasianinae and Tetraoninae), 7 and 12 (Phasianinae and Tetraoninae), 5 and 13 (Perdicinae), 22 (Alectoris). Our study shows that analysis of the genomes of several closely related species allows us to identify chromosomal rearrangements characteristic of individual evolutionary lines.

KW - Bacterial artificial chromosomes

KW - Coturnix japonica

KW - Dot plots

KW - Tetrao urogallus

KW - Tetrastes bonasia

UR - https://www.mendeley.com/catalogue/57a94e31-131f-3e51-a9d5-ea9d89f34a2e/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105004402171&origin=inward&txGid=ad5968c37d2d22e41406565cdecf4d22

U2 - 10.1159/000545036

DO - 10.1159/000545036

M3 - Article

C2 - 40068633

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

ER -