TY - JOUR

T1 - Time-Resolved Chemically Induced Dynamic Nuclear Polarization of Biologically Important Molecules

AU - Morozova, Olga B.

AU - Ivanov, Konstantin L.

N1 - © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2019/1/21

Y1 - 2019/1/21

N2 - In this work, we review the hyperpolarization technique named chemically induced dynamic nuclear polarization (CIDNP), focusing on the time-resolved variant of this method and its biological applications. We introduce the main principles of polarization formation in liquids at high magnetic fields, provided by the so-called spin sorting mechanism. Applications of CIDNP to studying fast reactions of short-lived free radicals of biologically important molecules are discussed, as well as the potential of the method to probe the structure and magnetic parameters of such radicals. We also explain the principles of protein CIDNP and discuss applications of time-resolved CIDNP to studies of protein structure and dynamics.

AB - In this work, we review the hyperpolarization technique named chemically induced dynamic nuclear polarization (CIDNP), focusing on the time-resolved variant of this method and its biological applications. We introduce the main principles of polarization formation in liquids at high magnetic fields, provided by the so-called spin sorting mechanism. Applications of CIDNP to studying fast reactions of short-lived free radicals of biologically important molecules are discussed, as well as the potential of the method to probe the structure and magnetic parameters of such radicals. We also explain the principles of protein CIDNP and discuss applications of time-resolved CIDNP to studies of protein structure and dynamics.

KW - biomolecules

KW - chemically induced dynamic nuclear polarization

KW - radicals

KW - spin hyperpolarization

KW - time-resolved studies

KW - RADICAL PAIRS

KW - INTRAMOLECULAR ELECTRON-TRANSFER

KW - NMR-SPECTROSCOPY

KW - MAGNETIC-FIELD DEPENDENCE

KW - WAHREND RASCHER RADIKALREAKTIONEN

KW - TRYPTOPHAN-TYROSINE PEPTIDE

KW - MODEL-FREE APPROACH

KW - AQUEOUS-SOLUTION

KW - LASER FLASH-PHOTOLYSIS

KW - REACTION CENTERS

KW - Free Radicals/chemistry

KW - Magnetics

KW - Magnetic Resonance Spectroscopy/methods

KW - Proteins/chemistry

KW - Time Factors

KW - Protein Conformation

UR - http://www.scopus.com/inward/record.url?scp=85057791452&partnerID=8YFLogxK

U2 - 10.1002/cphc.201800566

DO - 10.1002/cphc.201800566

M3 - Review article

C2 - 30328262

AN - SCOPUS:85057791452

VL - 20

SP - 197

EP - 215

JO - ChemPhysChem

JF - ChemPhysChem

SN - 1439-4235

IS - 2

ER -