TY - JOUR

T1 - Theoretical model of mitotic spindle microtubule growth for FRAP curve interpretation

AU - Omelyanchuk, Leonid V.

AU - Munzarova, Alina F.

N1 - Publisher Copyright: © 2017 The Author(s).

PY - 2017/2/24

Y1 - 2017/2/24

N2 - Background: Spindle FRAP curves depend on the kinetic parameters of microtubule polymerization and depolymerization. The empirical FRAP curve proposed earlier permits determination of only one such dynamic parameter, commonly called the "tubulin turnover". The aim of our study was to build a FRAP curve based on an already known kinetic model of microtubule growth. Results: A numerical expression that describes the distribution of polymerizing and depolymerizing microtubule ends as a function of four kinetic parameters is presented. In addition, a theoretical FRAP curve for the metaphase spindle is constructed using previously published dynamic parameters. Conclusion: The numerical expression we elaborated can replace the empirical FRAP curve described earlier for a spindle comprising fluorescently marked microtubules. The curve we generated fits well the experimental data.

AB - Background: Spindle FRAP curves depend on the kinetic parameters of microtubule polymerization and depolymerization. The empirical FRAP curve proposed earlier permits determination of only one such dynamic parameter, commonly called the "tubulin turnover". The aim of our study was to build a FRAP curve based on an already known kinetic model of microtubule growth. Results: A numerical expression that describes the distribution of polymerizing and depolymerizing microtubule ends as a function of four kinetic parameters is presented. In addition, a theoretical FRAP curve for the metaphase spindle is constructed using previously published dynamic parameters. Conclusion: The numerical expression we elaborated can replace the empirical FRAP curve described earlier for a spindle comprising fluorescently marked microtubules. The curve we generated fits well the experimental data.

KW - Fluorescently marked tubulin

KW - FRAP (fluorescence recovery after photobleaching)

KW - Growing/shrinking microtubule ends

KW - Microtubules

KW - Mitotic spindle

KW - PDE (partial differential equation)

KW - Fluorescence Recovery After Photobleaching

KW - Models, Biological

KW - Spindle Apparatus/metabolism

KW - Microtubules/metabolism

KW - FLUX

KW - ANAPHASE-B

KW - DYNAMICS

KW - SWITCH

KW - TUBULIN

UR - http://www.scopus.com/inward/record.url?scp=85013743375&partnerID=8YFLogxK

U2 - 10.1186/s12918-016-0378-9

DO - 10.1186/s12918-016-0378-9

M3 - Article

C2 - 28466790

AN - SCOPUS:85013743375

VL - 11

SP - 378

JO - BMC Systems Biology

JF - BMC Systems Biology

SN - 1752-0509

IS - Suppl 1

M1 - 3

ER -