Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins. / Kashirina, Daria N.; Percy, Andrew J.; Pastushkova, Liudmila Kh и др.
в: BMC Medical Genomics, Том 12, № Suppl 2, 45, 13.03.2019, стр. 45.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
AU - Kashirina, Daria N.
AU - Percy, Andrew J.
AU - Pastushkova, Liudmila Kh
AU - Borchers, Christoph H.
AU - Kireev, Kirill S.
AU - Ivanisenko, Vladimir A.
AU - Kononikhin, Alexey S.
AU - Nikolaev, Eugene N.
AU - Larina, Irina M.
PY - 2019/3/13
Y1 - 2019/3/13
N2 - Background: The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. Methods: Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station were collected 30 days before launch and on the first and seventh days after landing. Results: A panel of 125 proteins in the blood plasma was quantitated by a well-established and highly regarded targeted mass spectrometry approach. This method involves the monitoring of multiple reactions in conjunction with stable isotope-labeled standards at the University of Victoria - Genome BC Proteomics Centre. Conclusions: Reduction of circulating plasma volume during space flight and activation of fluid retention at the final stage of the flight affect the changes in plasma protein concentrations present in the first days after landing. Using an ANOVA approach, it was revealed that only 1 protein (S100A9) reliably responded to space flight conditions. This protein plays an important role in the functioning of the endothelium and can serve as a marker for activation of inflammatory reactions. Concentrations of the proteins of complement, coagulation cascades, and acute phase reactants increase in the blood of cosmonauts as measured the first day after landing. Most of these proteins' concentrations continue to increase by the 7th day after space flight. Similar dynamics are observed for proteases and their inhibitors. Thus, there is a shift in proteolytic blood systems, which is necessary for the restoration of muscle tissue and maintenance of oncotic homeostasis.
AB - Background: The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. Methods: Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station were collected 30 days before launch and on the first and seventh days after landing. Results: A panel of 125 proteins in the blood plasma was quantitated by a well-established and highly regarded targeted mass spectrometry approach. This method involves the monitoring of multiple reactions in conjunction with stable isotope-labeled standards at the University of Victoria - Genome BC Proteomics Centre. Conclusions: Reduction of circulating plasma volume during space flight and activation of fluid retention at the final stage of the flight affect the changes in plasma protein concentrations present in the first days after landing. Using an ANOVA approach, it was revealed that only 1 protein (S100A9) reliably responded to space flight conditions. This protein plays an important role in the functioning of the endothelium and can serve as a marker for activation of inflammatory reactions. Concentrations of the proteins of complement, coagulation cascades, and acute phase reactants increase in the blood of cosmonauts as measured the first day after landing. Most of these proteins' concentrations continue to increase by the 7th day after space flight. Similar dynamics are observed for proteases and their inhibitors. Thus, there is a shift in proteolytic blood systems, which is necessary for the restoration of muscle tissue and maintenance of oncotic homeostasis.
KW - Blood proteins
KW - Cosmonauts
KW - Mass spectrometry
KW - MONOCYTES
KW - SPACEFLIGHT
KW - IL-6
UR - http://www.scopus.com/inward/record.url?scp=85062938692&partnerID=8YFLogxK
U2 - 10.1186/s12920-019-0490-y
DO - 10.1186/s12920-019-0490-y
M3 - Article
C2 - 30871558
AN - SCOPUS:85062938692
VL - 12
SP - 45
JO - BMC Medical Genomics
JF - BMC Medical Genomics
SN - 1755-8794
IS - Suppl 2
M1 - 45
ER -
ID: 18860516