Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The Kinetic Mechanism of 3′-5′ Exonucleolytic Activity of AP Endonuclease Nfo from E. coli. / Senchurova, Svetlana I.; Kuznetsova, Aleksandra A.; Ishchenko, Alexander A. и др.
в: Cells, Том 11, № 19, 2998, 10.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - The Kinetic Mechanism of 3′-5′ Exonucleolytic Activity of AP Endonuclease Nfo from E. coli
AU - Senchurova, Svetlana I.
AU - Kuznetsova, Aleksandra A.
AU - Ishchenko, Alexander A.
AU - Saparbaev, Murat
AU - Fedorova, Olga S.
AU - Kuznetsov, Nikita A.
N1 - Funding Information: This work was supported by the Ministry of Science and Higher Education of the Russian Federation, agreement No. 075-15-2021-1085. Publisher Copyright: © 2022 by the authors.
PY - 2022/10
Y1 - 2022/10
N2 - Escherichia coli apurinic/apyrimidinic (AP) endonuclease Nfo is one of the key participants in DNA repair. The principal biological role of this enzyme is the recognition and hydrolysis of AP sites, which arise in DNA either as a result of the spontaneous hydrolysis of an N-glycosidic bond with intact nitrogenous bases or under the action of DNA glycosylases, which eliminate various damaged bases during base excision repair. Nfo also removes 3′-terminal blocking groups resulting from AP lyase activity of DNA glycosylases. Additionally, Nfo can hydrolyze the phosphodiester linkage on the 5′ side of some damaged nucleotides on the nucleotide incision repair pathway. The function of 3′-5′-exonuclease activity of Nfo remains unclear and probably consists of participation (together with the nucleotide incision repair activity) in the repair of cluster lesions. In this work, using polyacrylamide gel electrophoresis and the stopped-flow method, we analyzed the kinetics of the interaction of Nfo with various model DNA substrates containing a 5′ single-stranded region. These data helped to describe the mechanism of nucleotide cleavage and to determine the rates of the corresponding stages. It was revealed that the rate-limiting stage of the enzymatic process is a dissociation of the reaction product from the enzyme active site. The stability of the terminal pair of nucleotides in the substrate did not affect the enzymatic-reaction rate. Finally, it was found that 2′-deoxynucleoside monophosphates can effectively inhibit the 3′-5′-exonuclease activity of Nfo.
AB - Escherichia coli apurinic/apyrimidinic (AP) endonuclease Nfo is one of the key participants in DNA repair. The principal biological role of this enzyme is the recognition and hydrolysis of AP sites, which arise in DNA either as a result of the spontaneous hydrolysis of an N-glycosidic bond with intact nitrogenous bases or under the action of DNA glycosylases, which eliminate various damaged bases during base excision repair. Nfo also removes 3′-terminal blocking groups resulting from AP lyase activity of DNA glycosylases. Additionally, Nfo can hydrolyze the phosphodiester linkage on the 5′ side of some damaged nucleotides on the nucleotide incision repair pathway. The function of 3′-5′-exonuclease activity of Nfo remains unclear and probably consists of participation (together with the nucleotide incision repair activity) in the repair of cluster lesions. In this work, using polyacrylamide gel electrophoresis and the stopped-flow method, we analyzed the kinetics of the interaction of Nfo with various model DNA substrates containing a 5′ single-stranded region. These data helped to describe the mechanism of nucleotide cleavage and to determine the rates of the corresponding stages. It was revealed that the rate-limiting stage of the enzymatic process is a dissociation of the reaction product from the enzyme active site. The stability of the terminal pair of nucleotides in the substrate did not affect the enzymatic-reaction rate. Finally, it was found that 2′-deoxynucleoside monophosphates can effectively inhibit the 3′-5′-exonuclease activity of Nfo.
KW - apurinic/apyrimidinic endonuclease
KW - DNA repair
KW - fluorescence
KW - pre-steady-state kinetics
KW - DNA/metabolism
KW - DNA Glycosylases/genetics
KW - DNA-(Apurinic or Apyrimidinic Site) Lyase/metabolism
KW - Endonucleases/genetics
KW - Humans
KW - Nucleotides
KW - Escherichia coli/metabolism
KW - Exonucleases/genetics
KW - DNA Damage
UR - http://www.scopus.com/inward/record.url?scp=85140017574&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/ea58cc46-196b-3c27-b95b-afa359f20410/
U2 - 10.3390/cells11192998
DO - 10.3390/cells11192998
M3 - Article
C2 - 36230958
AN - SCOPUS:85140017574
VL - 11
JO - Cells
JF - Cells
SN - 2073-4409
IS - 19
M1 - 2998
ER -
ID: 38203288