TY - JOUR

T1 - The influence of zinc and silicate ions on biological properties of hydroxyapatite synthesized by a mechanochemical method

AU - Khvostov, Mikhail V.

AU - Borisova, Marina S.

AU - Bulina, Natalia V.

AU - Makarova, Svetlana V.

AU - Dumchenko, Natalya B.

AU - Tolstikova, Tatjana G.

AU - Lyakhov, Nikolay Z.

N1 - Publisher Copyright: © 2020 Elsevier Ltd and Techna Group S.r.l. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - In this study, a mechanochemical method was applied to the synthesis of hydroxyapatite with double and triple substitution. Simultaneous replacement of phosphate ions by carbonate and silicate ions was implemented in the case of double substitution. The triple substitution involved additional replacement of a calcium ion by a zinc ion. The synthesized materials were characterized by X-ray diffraction and Fourier transform infrared spectroscopy. Samples of co-substituted hydroxyapatites in powder form were evaluated in vitro using human osteoblast cell line MG-63 and in vivo via implantation into a bone defect in the rat cranial vault. After 4 days of incubation in vitro, the hydroxyapatite containing zinc, silicon, and carbonate ions with composition Ca9.9Zn0.1(PO4)5.6(SiO4)0.2(CO3)0.2(OH)2 significantly increased cell proliferation in comparison with the other compositions. Four months after the implantation in vivo, this sample showed the greatest ability to integrate into rat skull bones, as evidenced by a morphological analysis of bone sections.

AB - In this study, a mechanochemical method was applied to the synthesis of hydroxyapatite with double and triple substitution. Simultaneous replacement of phosphate ions by carbonate and silicate ions was implemented in the case of double substitution. The triple substitution involved additional replacement of a calcium ion by a zinc ion. The synthesized materials were characterized by X-ray diffraction and Fourier transform infrared spectroscopy. Samples of co-substituted hydroxyapatites in powder form were evaluated in vitro using human osteoblast cell line MG-63 and in vivo via implantation into a bone defect in the rat cranial vault. After 4 days of incubation in vitro, the hydroxyapatite containing zinc, silicon, and carbonate ions with composition Ca9.9Zn0.1(PO4)5.6(SiO4)0.2(CO3)0.2(OH)2 significantly increased cell proliferation in comparison with the other compositions. Four months after the implantation in vivo, this sample showed the greatest ability to integrate into rat skull bones, as evidenced by a morphological analysis of bone sections.

KW - Co-substituted hydroxyapatite

KW - Implantation

KW - In vivo

KW - MG63

KW - Osseointegration

KW - Silicon

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=85097660763&partnerID=8YFLogxK

U2 - 10.1016/j.ceramint.2020.12.083

DO - 10.1016/j.ceramint.2020.12.083

M3 - Article

AN - SCOPUS:85097660763

VL - 47

SP - 9495

EP - 9503

JO - Ceramics International

JF - Ceramics International

SN - 0272-8842

IS - 7

ER -