TY - JOUR

T1 - The Impact of a Cypovirus on Parental and Filial Generations of Lymantria dispar L

AU - Akhanaev, Yuriy B

AU - Pavlushin, Sergey V

AU - Kharlamova, Daria D

AU - Odnoprienko, Daria

AU - Subbotina, Anna O

AU - Belousova, Irina A

AU - Ignatieva, Anastasia N

AU - Kononchuk, Anastasia G

AU - Tokarev, Yuri S

AU - Martemyanov, Vyacheslav V

N1 - This research was funded by the Russian Science Foundation, grant number 21-46-07005.

PY - 2023/12

Y1 - 2023/12

N2 - Recently, we found that the spongy moth Lymantria dispar L. is susceptible to infection by a Dendrolimus sibiricus cytoplasmic polyhedrosis virus (DsCPV-1). In the present study, we evaluated the pathogenicity of DsCPV-1 against L. dispar larvae and its impact on surviving insects after the infection. Offspring of virally challenged insects were tested for susceptibility to a stress factor (starvation). In addition, we used light microscopy and quantitative polymerase chain reaction (qPCR) to test the ability of DsCPV-1 to be transmitted vertically. We found insect mortality of the L. dispar parents following the infection was positively associated with DsCPV-1 dose. DsCPV-1 was lethal to second-instar L. dispar larvae with a 50% lethal dose (LD50) of 1687 occlusion bodies per larva. No vertical transmission of DsCPV-1 to offspring larvae was detected, while the majority of insect deaths among offspring larvae were caused by microsporidia (Vairimorpha lymantriae), which was harbored by the parents. The offspring of virally challenged parents exhibited a higher number of detected microsporidia compared to the control. Our findings suggest that the application of DsCPV-1 is effective in controlling pests in terms of transgenerational impact following virus exposure.

AB - Recently, we found that the spongy moth Lymantria dispar L. is susceptible to infection by a Dendrolimus sibiricus cytoplasmic polyhedrosis virus (DsCPV-1). In the present study, we evaluated the pathogenicity of DsCPV-1 against L. dispar larvae and its impact on surviving insects after the infection. Offspring of virally challenged insects were tested for susceptibility to a stress factor (starvation). In addition, we used light microscopy and quantitative polymerase chain reaction (qPCR) to test the ability of DsCPV-1 to be transmitted vertically. We found insect mortality of the L. dispar parents following the infection was positively associated with DsCPV-1 dose. DsCPV-1 was lethal to second-instar L. dispar larvae with a 50% lethal dose (LD50) of 1687 occlusion bodies per larva. No vertical transmission of DsCPV-1 to offspring larvae was detected, while the majority of insect deaths among offspring larvae were caused by microsporidia (Vairimorpha lymantriae), which was harbored by the parents. The offspring of virally challenged parents exhibited a higher number of detected microsporidia compared to the control. Our findings suggest that the application of DsCPV-1 is effective in controlling pests in terms of transgenerational impact following virus exposure.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180679912&origin=inward&txGid=33febf068adfc73ad755a21e147a55cd

U2 - 10.3390/insects14120917

DO - 10.3390/insects14120917

M3 - Article

C2 - 38132591

VL - 14

JO - Insects

JF - Insects

SN - 2075-4450

IS - 12

M1 - 917

ER -