Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The effect of vasopressin on the expression of genes of key enzymes of the interstitial hyaluronan turnover and concentration ability in WAG rat kidneys. / Ivanova, L. N.; Babina, A. V.; Baturina, G. S. и др.
в: Russian Journal of Genetics: Applied Research, Том 7, № 3, 01.05.2017, стр. 249-257.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - The effect of vasopressin on the expression of genes of key enzymes of the interstitial hyaluronan turnover and concentration ability in WAG rat kidneys
AU - Ivanova, L. N.
AU - Babina, A. V.
AU - Baturina, G. S.
AU - Katkova, L. E.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - In mammals arginine-vasopressin (AVP) is a major hormone involved in the regulation of renal water reabsorption, acting via the increase of the osmotic permeability of the epithelium of the collecting duct. The AVP-induced intracellular events include, as a core step, the trafficking of the vesicles containing the water channels, aquaporin-2, to the apical plasma membrane of the collecting duct’s principal cells. The interstitium of the renal inner medulla contains abundant linear negatively charged glycosaminoglycan, hyaluronan (HA), which affects the water flow between structures of the concentrating mechanism, depending on its polymeric state. Using real-time RT-PCR, we tested the assumption that the renal hyaluronan may be involved in the long-term vasopressin effect on water reabsorption. The expression of the genes encoding hyaluronan synthase-2 (Has2), hyaluronidase-1, and hyaluronidase-2 (Hyal1 and Hyal2) in the kidneys of Wistar Albino Glaxo (WAG) was studied. The Has2 mRNA content was the highest in the papilla of the kidneys of the hydrated rats. The V2 receptor-selective vasopressin analog dDAVP (100 μg/kg of body weight, twice a day intraperitoneally for 2 days) induced a considerable decrease in the Has2 mRNA content in the papilla with less pronounced changes in the cortex. In contrast to Has2, the dDAVP treatment caused a significant increase in the Hyal1 and Hyal2 mRNA content in the renal papilla. There was a good fit between the Hyal1 and Hyal2 transcriptional levels and changes in hyaluronidase activity in the renal tissue. It was suggested that vasopressin is able to inhibit the synthesis of hyaluronan and concomitantly promotes its degradation in the renal papilla interstitium, thereby facilitating water flow between the elements of the renal countercurrent system. The implications for this effect are discussed in the context of the data in the literature.
AB - In mammals arginine-vasopressin (AVP) is a major hormone involved in the regulation of renal water reabsorption, acting via the increase of the osmotic permeability of the epithelium of the collecting duct. The AVP-induced intracellular events include, as a core step, the trafficking of the vesicles containing the water channels, aquaporin-2, to the apical plasma membrane of the collecting duct’s principal cells. The interstitium of the renal inner medulla contains abundant linear negatively charged glycosaminoglycan, hyaluronan (HA), which affects the water flow between structures of the concentrating mechanism, depending on its polymeric state. Using real-time RT-PCR, we tested the assumption that the renal hyaluronan may be involved in the long-term vasopressin effect on water reabsorption. The expression of the genes encoding hyaluronan synthase-2 (Has2), hyaluronidase-1, and hyaluronidase-2 (Hyal1 and Hyal2) in the kidneys of Wistar Albino Glaxo (WAG) was studied. The Has2 mRNA content was the highest in the papilla of the kidneys of the hydrated rats. The V2 receptor-selective vasopressin analog dDAVP (100 μg/kg of body weight, twice a day intraperitoneally for 2 days) induced a considerable decrease in the Has2 mRNA content in the papilla with less pronounced changes in the cortex. In contrast to Has2, the dDAVP treatment caused a significant increase in the Hyal1 and Hyal2 mRNA content in the renal papilla. There was a good fit between the Hyal1 and Hyal2 transcriptional levels and changes in hyaluronidase activity in the renal tissue. It was suggested that vasopressin is able to inhibit the synthesis of hyaluronan and concomitantly promotes its degradation in the renal papilla interstitium, thereby facilitating water flow between the elements of the renal countercurrent system. The implications for this effect are discussed in the context of the data in the literature.
KW - 2
KW - gene expression
KW - hyaluronan
KW - hyaluronansynthase-2
KW - hyaluronidase-1
KW - kidney
KW - vasopressin
KW - water reabsorption
UR - http://www.scopus.com/inward/record.url?scp=85018981326&partnerID=8YFLogxK
U2 - 10.1134/S2079059717030066
DO - 10.1134/S2079059717030066
M3 - Article
AN - SCOPUS:85018981326
VL - 7
SP - 249
EP - 257
JO - Russian Journal of Genetics: Applied Research
JF - Russian Journal of Genetics: Applied Research
SN - 2079-0597
IS - 3
ER -
ID: 10194039