TY - JOUR

T1 - The Effect of Dietary Phospholipids on the Ultrastructure and Function of Intestinal Epithelial Cells

AU - Saydakova, Snezhanna

AU - Morozova, Ksenia

AU - Snytnikova, Olga

AU - Morozova, Maryana

AU - Boldyreva, Lidiya

AU - Kiseleva, Elena

AU - Tsentalovich, Yuri

AU - Kozhevnikova, Elena

N1 - Funding: This research was funded by the Russian Science Foundation, grant number 20-74-10022.

PY - 2023/1/16

Y1 - 2023/1/16

N2 - Dietary composition substantially determines human health and affects complex diseases, including obesity, inflammation and cancer. Thus, food supplements have been widely used to accommodate dietary composition to the needs of individuals. Among the promising supplements are dietary phospholipids (PLs) that are commonly found as natural food ingredients and as emulsifier additives. The aim of the present study was to evaluate the effect of major PLs found as food supplements on the morphology of intestinal epithelial cells upon short-term and long-term high-dose feeding in mice. In the present report, the effect of short-term and long-term high dietary PL content was studied in terms of intestinal health and leaky gut syndrome in male mice. We used transmission electron microscopy to evaluate endothelial morphology at the ultrastructural level. We found mitochondrial damage and lipid droplet accumulation in the intracristal space, which rendered mitochondria more sensitive to respiratory uncoupling as shown by a mitochondrial respiration assessment in the intestinal crypts. However, this mitochondrial damage was insufficient to induce intestinal permeability. We propose that high-dose PL treatment impairs mitochondrial morphology and acts through extensive membrane utilization via the mitochondria. The data suggest that PL supplementation should be used with precaution in individuals with mitochondrial disorders.

AB - Dietary composition substantially determines human health and affects complex diseases, including obesity, inflammation and cancer. Thus, food supplements have been widely used to accommodate dietary composition to the needs of individuals. Among the promising supplements are dietary phospholipids (PLs) that are commonly found as natural food ingredients and as emulsifier additives. The aim of the present study was to evaluate the effect of major PLs found as food supplements on the morphology of intestinal epithelial cells upon short-term and long-term high-dose feeding in mice. In the present report, the effect of short-term and long-term high dietary PL content was studied in terms of intestinal health and leaky gut syndrome in male mice. We used transmission electron microscopy to evaluate endothelial morphology at the ultrastructural level. We found mitochondrial damage and lipid droplet accumulation in the intracristal space, which rendered mitochondria more sensitive to respiratory uncoupling as shown by a mitochondrial respiration assessment in the intestinal crypts. However, this mitochondrial damage was insufficient to induce intestinal permeability. We propose that high-dose PL treatment impairs mitochondrial morphology and acts through extensive membrane utilization via the mitochondria. The data suggest that PL supplementation should be used with precaution in individuals with mitochondrial disorders.

KW - Male

KW - Humans

KW - Mice

KW - Animals

KW - Phospholipids/pharmacology

KW - Diet

KW - Dietary Supplements

KW - Mitochondria

KW - Glycerophospholipids

KW - Fatty Acids/pharmacology

KW - Epithelial Cells

KW - intestinal barrier

KW - mitochondria

KW - phospholipids

KW - metabolism

KW - diet

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85146661416&origin=inward&txGid=3611822a4478f2c19245546691128648

UR - https://www.mendeley.com/catalogue/59074b57-1559-3be1-8c5a-8d7de6a0edab/

U2 - 10.3390/ijms24021788

DO - 10.3390/ijms24021788

M3 - Article

C2 - 36675301

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 2

M1 - 1788

ER -