Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Template Properties of 5-Methyl-2'-Deoxycytidine and 5-Hydroxymethyl-2'-Deoxycytidine in Reactions with Human Translesion and Reparative DNA Polymerases. / Shilkin, E. S.; Petrova, D. V.; Poltorachenko, V. A. и др.
в: Molecular Biology, Том 55, № 2, 03.2021, стр. 267-272.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Template Properties of 5-Methyl-2'-Deoxycytidine and 5-Hydroxymethyl-2'-Deoxycytidine in Reactions with Human Translesion and Reparative DNA Polymerases
AU - Shilkin, E. S.
AU - Petrova, D. V.
AU - Poltorachenko, V. A.
AU - Boldinova, E. O.
AU - Zharkov, D. O.
AU - Makarova, A. V.
N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (project nos. 17-00-00264-komfi (AVM) and 17-00-00261-komfi (DOZ)). PrimPol activity testing was supported by the Russian Science Foundation (project no. 18-14-00354 (AVM)). Publisher Copyright: © 2021, Pleiades Publishing, Inc. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3
Y1 - 2021/3
N2 - 5-Methyl-2'-deoxycytidine (mC) and the product of its controlled oxidation, 5-hydroxymethyl-2'-cytidine (hmC), play a key role in the epigenetic regulation of gene expression, the cell differentiation, and the carcinogenesis. Due to spontaneious deamination, genomic CpG sites containing mC and hmC serve as mutagenesis hotspots. In addition, error-prone translesion and reparative DNA polymerases may serve as additional source of mutations in the lesion-containing regions with CpG sites. In the present work, we performed in vitro analysis of the accuracy of nucleotide incorporation opposite to mC and hmC by human DNA polymerases Polβ, Polλ, Polη, Polι, Polκ and primase polymerase PrimPol. The results of the study show a high accuracy of copying mC and hmC by the reparative DNA polymerases Polβ and Polλ, while Polη, Polι, Polκ, and PrimPol copied mC and hmC with less accuracy evident by incorporation of dAMP and dTMP. The same spectrum of error-prone dNMP incorporation was also noted at sites with unmodified cytosines.
AB - 5-Methyl-2'-deoxycytidine (mC) and the product of its controlled oxidation, 5-hydroxymethyl-2'-cytidine (hmC), play a key role in the epigenetic regulation of gene expression, the cell differentiation, and the carcinogenesis. Due to spontaneious deamination, genomic CpG sites containing mC and hmC serve as mutagenesis hotspots. In addition, error-prone translesion and reparative DNA polymerases may serve as additional source of mutations in the lesion-containing regions with CpG sites. In the present work, we performed in vitro analysis of the accuracy of nucleotide incorporation opposite to mC and hmC by human DNA polymerases Polβ, Polλ, Polη, Polι, Polκ and primase polymerase PrimPol. The results of the study show a high accuracy of copying mC and hmC by the reparative DNA polymerases Polβ and Polλ, while Polη, Polι, Polκ, and PrimPol copied mC and hmC with less accuracy evident by incorporation of dAMP and dTMP. The same spectrum of error-prone dNMP incorporation was also noted at sites with unmodified cytosines.
KW - 5-hydroxymethylcytosine
KW - 5-methylcytosine
KW - CpG sites
KW - mutagenesis
KW - translesion DNA synthesis
UR - http://www.scopus.com/inward/record.url?scp=85105029365&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=44675047
U2 - 10.1134/S0026893321020138
DO - 10.1134/S0026893321020138
M3 - Article
AN - SCOPUS:85105029365
VL - 55
SP - 267
EP - 272
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 2
ER -
ID: 28498480