Результаты исследований: Научные публикации в периодических изданиях › обзорная статья › Рецензирование
Systems of Delivery of CRISPR/Cas9 Ribonucleoprotein Complexes for Genome Editing. / Amirkhanov, R. N.; Stepanov, G. A.
в: Russian Journal of Bioorganic Chemistry, Том 45, № 6, 01.11.2019, стр. 431-437.Результаты исследований: Научные публикации в периодических изданиях › обзорная статья › Рецензирование
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TY - JOUR
T1 - Systems of Delivery of CRISPR/Cas9 Ribonucleoprotein Complexes for Genome Editing
AU - Amirkhanov, R. N.
AU - Stepanov, G. A.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - The discovery of RNA-guided nucleases have enabled to leap forward in genome editing of cells and organisms. These nucleases can be delivered into cells as plasmid DNA, mRNA or ribonucleoprotein complexes (RNPs). The delivery in the form of RNP has some advantages because the target gene editing begins immediately without the process of intracellular synthesis of components and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR associated protein 9) system assembly. This strategy makes it possible to directly control RNP concentration and to decrease the number of off-targets due to rapid degradation of the complex in the cell. However, the task to develop RNP delivery systems remains unsolved. This review is devoted to RNP delivery into cells and tissues using physical approaches and different carriers. Special attention is paid to novel approaches that improve the RNP delivery efficiency.
AB - The discovery of RNA-guided nucleases have enabled to leap forward in genome editing of cells and organisms. These nucleases can be delivered into cells as plasmid DNA, mRNA or ribonucleoprotein complexes (RNPs). The delivery in the form of RNP has some advantages because the target gene editing begins immediately without the process of intracellular synthesis of components and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR associated protein 9) system assembly. This strategy makes it possible to directly control RNP concentration and to decrease the number of off-targets due to rapid degradation of the complex in the cell. However, the task to develop RNP delivery systems remains unsolved. This review is devoted to RNP delivery into cells and tissues using physical approaches and different carriers. Special attention is paid to novel approaches that improve the RNP delivery efficiency.
KW - CRISPR/Cas9
KW - delivery
KW - genome editing
KW - ribonucleoprotein complexes
UR - http://www.scopus.com/inward/record.url?scp=85078604527&partnerID=8YFLogxK
U2 - 10.1134/S1068162019060025
DO - 10.1134/S1068162019060025
M3 - Review article
AN - SCOPUS:85078604527
VL - 45
SP - 431
EP - 437
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 6
ER -
ID: 23570221