Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Synergism of the Cytopathic Effect of Synchrotron Radiation and Manganese Oxide Nanoparticles on the Growth of Human Glioblastoma Cells In Vitro. / Razumov, I. A.; Troitsky, S. Yu; Zavjalov, E. L. и др.
в: Nanotechnologies in Russia, Том 15, № 11-12, 11.2020, стр. 819-827.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Synergism of the Cytopathic Effect of Synchrotron Radiation and Manganese Oxide Nanoparticles on the Growth of Human Glioblastoma Cells In Vitro
AU - Razumov, I. A.
AU - Troitsky, S. Yu
AU - Zavjalov, E. L.
AU - Solovieva, O. I.
AU - Goldenberg, B. G.
AU - Legkodymov, A. A.
AU - Lemzyakov, A. G.
AU - Kuper, K. E.
N1 - Funding Information: These studies were supported by Ministry of Science and Higher Education of Russian Federation (project no. 0324-2019-0041, unique project identifier RFMEFI62119X0023). Publisher Copyright: © 2020, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020/11
Y1 - 2020/11
N2 - A binary cytopathic effect (CPE%) of synchrotron radiation (SR) and manganese oxide nanoparticles (MnO NP) on human glioblastoma U87MG, U-251MG, and A-172 cell lines was studied in vitro. After incubation of the cells with MnO NP at a concentration of 5 and 50 µmol for 18–24 h, irradiation of glioblastoma cells at the source of SR VEPP-4 was carried out. X-ray irradiation doses 0, 1, 2, 4 Gy were used. The results were estimated using a light microscopy, determination of CPE% on the counter (Countess, Invitrogen), and the clonogenic test. It was revealed that the preliminary incubation of glioblastoma cell lines with MnO NP causes an increase in CPE of external SR at a dose 1, 2, and 4 Gy on the glioblastoma cells by 25, 50, and 100%. This effect allows to reduce the radiation dose by 2–3 times, maintaining the efficiency of the effect. At the same time, the period of vital activity of tumor cells under a combined effect of SR and NP is reduced by 18–36 h. Probably, a synergism of the combination of MnO NP and SR will provide new opportunities for the development of a model for the treatment of brain tumors.
AB - A binary cytopathic effect (CPE%) of synchrotron radiation (SR) and manganese oxide nanoparticles (MnO NP) on human glioblastoma U87MG, U-251MG, and A-172 cell lines was studied in vitro. After incubation of the cells with MnO NP at a concentration of 5 and 50 µmol for 18–24 h, irradiation of glioblastoma cells at the source of SR VEPP-4 was carried out. X-ray irradiation doses 0, 1, 2, 4 Gy were used. The results were estimated using a light microscopy, determination of CPE% on the counter (Countess, Invitrogen), and the clonogenic test. It was revealed that the preliminary incubation of glioblastoma cell lines with MnO NP causes an increase in CPE of external SR at a dose 1, 2, and 4 Gy on the glioblastoma cells by 25, 50, and 100%. This effect allows to reduce the radiation dose by 2–3 times, maintaining the efficiency of the effect. At the same time, the period of vital activity of tumor cells under a combined effect of SR and NP is reduced by 18–36 h. Probably, a synergism of the combination of MnO NP and SR will provide new opportunities for the development of a model for the treatment of brain tumors.
UR - http://www.scopus.com/inward/record.url?scp=85105487391&partnerID=8YFLogxK
U2 - 10.1134/S1995078020060166
DO - 10.1134/S1995078020060166
M3 - Article
AN - SCOPUS:85105487391
VL - 15
SP - 819
EP - 827
JO - Nanotechnologies in Russia
JF - Nanotechnologies in Russia
SN - 1995-0780
IS - 11-12
ER -
ID: 28563731