TY - JOUR

T1 - Surgical technique for explantation of a functioning cardiopulmonary complex in an experiment

AU - Zhulkov, M. O.

AU - Zykov, I. S.

AU - Makaev, A. G.

AU - Protopopov, A. V.

AU - Murtazaliev, M. N.

AU - Kosimov, F. Yu.

AU - Tarkova, A. R.

AU - Limansky, A. D.

AU - Smirnov, Ya. M.

AU - Agaeva, H. A.

AU - Frykina, O. E.

AU - Sirota, D. A.

N1 - Публикация для корректировки.

PY - 2023

Y1 - 2023

N2 - Objective : to develop and approve the surgical technique for explantation of a functioning cardiopulmonary complex under normothermic autoperfusion. Materials and methods . Landrace pigs were used as the experimental model for a series of acute experiments (n = 10). During the experiment, invasive pressure in the cavities of the heart and main arteries, blood gas composition, and myocardial contractility were monitored. The functioning cardiopulmonary complex was explanted through a median sternotomy. The explanted complex was conditioned at 37–38 °C for 6 hours. Results . In the course of a series of experiments, it was shown that stable operation of the isolated heart-lung complex ex vivo for 6 hours was fundamentally possible provided that the parameters of the basic homeostasis constants are maintained. The technological solutions used made it possible to ensure safe hemodynamic and anatomical isolation of the working cardiopulmonary complex. Conclusion . The developed protocol for isolating a functioning cardiopulmonary complex allows to provide stable graft function for 6 hours under normothermic autoperfusion. Implementation of this concept in the development of transport systems would significantly facilitate their design and eliminate the use of expensive components. This would contribute to widespread introduction into clinical practice.

AB - Objective : to develop and approve the surgical technique for explantation of a functioning cardiopulmonary complex under normothermic autoperfusion. Materials and methods . Landrace pigs were used as the experimental model for a series of acute experiments (n = 10). During the experiment, invasive pressure in the cavities of the heart and main arteries, blood gas composition, and myocardial contractility were monitored. The functioning cardiopulmonary complex was explanted through a median sternotomy. The explanted complex was conditioned at 37–38 °C for 6 hours. Results . In the course of a series of experiments, it was shown that stable operation of the isolated heart-lung complex ex vivo for 6 hours was fundamentally possible provided that the parameters of the basic homeostasis constants are maintained. The technological solutions used made it possible to ensure safe hemodynamic and anatomical isolation of the working cardiopulmonary complex. Conclusion . The developed protocol for isolating a functioning cardiopulmonary complex allows to provide stable graft function for 6 hours under normothermic autoperfusion. Implementation of this concept in the development of transport systems would significantly facilitate their design and eliminate the use of expensive components. This would contribute to widespread introduction into clinical practice.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85175424841&origin=inward&txGid=72ae3de4d6b89b0c7e920b9c363d378d

UR - https://www.elibrary.ru/item.asp?id=54370937

UR - https://www.mendeley.com/catalogue/f9673ceb-3038-3dea-9c85-359f23f7b536/

U2 - 10.15825/1995-1191-2023-3-122-128

DO - 10.15825/1995-1191-2023-3-122-128

M3 - Article

VL - 25

SP - 122

EP - 128

JO - Вестник трансплантологии и искусственных органов

JF - Вестник трансплантологии и искусственных органов

SN - 1995-1191

IS - 3

ER -