Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Supporting effect of polyethylenimine on hexarhenium hydroxo cluster complex for cellular imaging applications. / Elistratova, Julia G.; Brylev, Konstantin A.; Solovieva, Anastasiya O. и др.
в: Journal of Photochemistry and Photobiology A: Chemistry, Том 340, 01.05.2017, стр. 46-52.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Supporting effect of polyethylenimine on hexarhenium hydroxo cluster complex for cellular imaging applications
AU - Elistratova, Julia G.
AU - Brylev, Konstantin A.
AU - Solovieva, Anastasiya O.
AU - Pozmogova, Tatyana N.
AU - Mustafina, Asiya R.
AU - Shestopalova, Lidiya V.
AU - Shestopalov, Michael A.
AU - Syakayev, Viktor V.
AU - Karasik, Andrey A.
AU - Sinyashin, Oleg G.
N1 - Publisher Copyright: © 2017
PY - 2017/5/1
Y1 - 2017/5/1
N2 - The present work introduces an insight into pH- and concentration-dependent binding of branched polyethylenimine (PEI) with hexarhenium hydroxo cluster complex [{Re6S8}(OH)6]4− aimed at enhancing the stability, luminescence intensity and cellular uptake of the cluster complex in aqueous solutions at pH 7.4. Luminescence and conductivity measurements revealed pH 7.4 and 1:1 of PEI:cluster molar ratio as specific conditions ensuring multiple electrostatic interactions of protonated amino groups of PEI with the tetra-anionic cluster units. These interactions resulted in a significant supporting effect of PEI on stability and luminescence of the cluster complex, while no effect of the cluster complex on the acid-base and aggregation properties of PEI was detected from dynamic light scattering and 1H NMR spectroscopy measurements. The cluster luminescence remained without degradation for at least two weeks due to the supporting effect of PEI, which can facilitate the application of the cluster complex in bioimaging. The cellular uptake of [{Re6S8}(OH)6]4− and [{Re6S8}(OH)6]4−@PEI was investigated. Confocal microscopic studies showed that the pristine cluster complex [{Re6S8}(OH)6]4− did not enter cells (the human larynx carcinoma cell line (Hep-2)), whereas the supramolecular assembly [{Re6S8}(OH)6]4−@PEI internalized into the cells.
AB - The present work introduces an insight into pH- and concentration-dependent binding of branched polyethylenimine (PEI) with hexarhenium hydroxo cluster complex [{Re6S8}(OH)6]4− aimed at enhancing the stability, luminescence intensity and cellular uptake of the cluster complex in aqueous solutions at pH 7.4. Luminescence and conductivity measurements revealed pH 7.4 and 1:1 of PEI:cluster molar ratio as specific conditions ensuring multiple electrostatic interactions of protonated amino groups of PEI with the tetra-anionic cluster units. These interactions resulted in a significant supporting effect of PEI on stability and luminescence of the cluster complex, while no effect of the cluster complex on the acid-base and aggregation properties of PEI was detected from dynamic light scattering and 1H NMR spectroscopy measurements. The cluster luminescence remained without degradation for at least two weeks due to the supporting effect of PEI, which can facilitate the application of the cluster complex in bioimaging. The cellular uptake of [{Re6S8}(OH)6]4− and [{Re6S8}(OH)6]4−@PEI was investigated. Confocal microscopic studies showed that the pristine cluster complex [{Re6S8}(OH)6]4− did not enter cells (the human larynx carcinoma cell line (Hep-2)), whereas the supramolecular assembly [{Re6S8}(OH)6]4−@PEI internalized into the cells.
KW - Cellular imaging
KW - Cytotoxicity
KW - Hexarhenium cluster
KW - Luminescence
KW - Polyethylenimine
KW - NANOPARTICLES
KW - SE
KW - MOLYBDENUM
KW - LUMINESCENT
KW - CHITOSAN
KW - SYSTEMS
KW - CYTOTOXICITY
UR - http://www.scopus.com/inward/record.url?scp=85014836181&partnerID=8YFLogxK
U2 - 10.1016/j.jphotochem.2017.03.005
DO - 10.1016/j.jphotochem.2017.03.005
M3 - Article
AN - SCOPUS:85014836181
VL - 340
SP - 46
EP - 52
JO - Journal of Photochemistry and Photobiology A: Chemistry
JF - Journal of Photochemistry and Photobiology A: Chemistry
SN - 1010-6030
ER -
ID: 9029469