Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Studying Structure and Functions of Nucleosomes with Atomic Force Microscopy. / Ukraintsev, Alexander A.; Kutuzov, Mikhail M.; Lavrik, Olga I.
в: Biochemistry (Moscow), Том 89, № 4, 04.2024, стр. 674-687.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Studying Structure and Functions of Nucleosomes with Atomic Force Microscopy
AU - Ukraintsev, Alexander A.
AU - Kutuzov, Mikhail M.
AU - Lavrik, Olga I.
PY - 2024/4
Y1 - 2024/4
N2 - Abstract: Chromatin is an epigenetic platform for implementation of DNA-dependent processes. Nucleosome, as a basic level of chromatin compaction, largely determines its properties and structure. In the study of nucleosomes structure and functions physicochemical tools are actively used, such as magnetic and optical “tweezers”, “DNA curtains”, nuclear magnetic resonance, X-ray crystallography, and cryogenic electron microscopy, as well as optical methods based on Förster resonance energy transfer. Despite the fact that these approaches make it possible to determine a wide range of structural and functional characteristics of chromatin and nucleosomes with high spatial and time resolution, atomic force microscopy (AFM) complements the capabilities of these methods. The results of structural studies of nucleosome focusing on the AFM method development are presented in this review. The possibilities of AFM are considered in the context of application of other physicochemical approaches.
AB - Abstract: Chromatin is an epigenetic platform for implementation of DNA-dependent processes. Nucleosome, as a basic level of chromatin compaction, largely determines its properties and structure. In the study of nucleosomes structure and functions physicochemical tools are actively used, such as magnetic and optical “tweezers”, “DNA curtains”, nuclear magnetic resonance, X-ray crystallography, and cryogenic electron microscopy, as well as optical methods based on Förster resonance energy transfer. Despite the fact that these approaches make it possible to determine a wide range of structural and functional characteristics of chromatin and nucleosomes with high spatial and time resolution, atomic force microscopy (AFM) complements the capabilities of these methods. The results of structural studies of nucleosome focusing on the AFM method development are presented in this review. The possibilities of AFM are considered in the context of application of other physicochemical approaches.
KW - AFM
KW - chromatin
KW - nucleosome
KW - single-molecule methods for studying biomolecules
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85192209552&origin=inward&txGid=a4162d58c831f7b60fe2a54edabd2df6
UR - https://www.mendeley.com/catalogue/16993b89-c6a9-3dd3-ae5e-535debb67f00/
U2 - 10.1134/S0006297924040072
DO - 10.1134/S0006297924040072
M3 - Article
C2 - 38831504
VL - 89
SP - 674
EP - 687
JO - Biochemistry (Moscow)
JF - Biochemistry (Moscow)
SN - 0006-2979
IS - 4
ER -
ID: 61084107