Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors. / Serdyukov, Danil S.; Goryachkovskaya, Tatiana N.; Mescheryakova, Irina A. и др.
в: Biomedical Optics Express, Том 11, № 9, 01.09.2020, стр. 5258-5273.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors
AU - Serdyukov, Danil S.
AU - Goryachkovskaya, Tatiana N.
AU - Mescheryakova, Irina A.
AU - Bannikova, Svetlana V.
AU - Kuznetsov, Sergei A.
AU - Cherkasova, Olga P.
AU - Popik, Vasiliy M.
AU - Peltek, Sergey E.
N1 - Publisher Copyright: © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement Copyright: Copyright 2021 Elsevier B.V., All rights reserved. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of Escherichia coli (E. coli) cells with plasmids, in which a promotor of genes matA, safA, or chbB controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters.
AB - Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of Escherichia coli (E. coli) cells with plasmids, in which a promotor of genes matA, safA, or chbB controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters.
KW - ACID RESISTANCE
KW - EXPRESSION
KW - OPERON
KW - PROTEINS
KW - ADHESION
KW - CASCADE
KW - CELLS
KW - WATER
KW - THZ
UR - http://www.scopus.com/inward/record.url?scp=85096230589&partnerID=8YFLogxK
U2 - 10.1364/BOE.400432
DO - 10.1364/BOE.400432
M3 - Article
C2 - 33014613
AN - SCOPUS:85096230589
VL - 11
SP - 5258
EP - 5273
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 9
ER -
ID: 26064625