TY - JOUR

T1 - Structural modeling of NAD+ binding modes to PARP-1

AU - Ivanisenko, N. V.

AU - Zhechev, D. A.

AU - Ivanisenko, V. A.

N1 - Publisher Copyright: © 2017, Pleiades Publishing, Ltd.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - The nuclear protein poly (ADP-ribose) polymerase-1 (PARP-1) plays an important role in the signaling and repair of DNA. PARP-1 catalyses the covalent binding of poly (ADP-ribose) polymers to its subunit, as well as to other acceptor proteins, using NAD+ as a donor of ADP-ribose. Inhibitors of poly (ADP-ribose) polymerase have been shown to be effective in improving radiation therapy and chemotherapy of cancer in clinical testing. Development of new poly (ADP-ribose) polymerase-1 inhibitors based on derivatives of natural compounds such as NAD+ represents a novel and promising strategy. The structure of the complex of human poly (ADP-ribose) polymerase-1 with NAD+ can be a starting point for the rational design of small molecule inhibitors based on NAD+ derivatives. Moreover, there is no crystal structure of the complex of poly (ADP-ribose) polymerase-1 with nicotinamide adenine dinucleotide (NAD+) available yet. In this work, using molecular modeling approaches, we have predicted NAD+ binding modes to PARP-1 at the donor binding site of the catalytic domain. Using structures of PARP-1 homologs in a complex with NAD+, we predict the pharmacophore restraints of NAD+ binding to PARP-1. Based on the clustering of PARP-1 conformations in a complex with cocrystallized inhibitors and the predicted pharmacophore restraints, we propose several possible models of NAD+ binding to PARP-1 at the donor binding site of the catalytic domain. According to the predicted models, two conformations for the pyrophosphate group of NAD+ in complex with PARP-1 at the donor binding site are possible. The proposed models of NAD+ binding to PARP-1 can be validated by the quantitative structure–activity analysis of NAD+ derivatives. We designed two NAD+ derivatives, which can be used to validate the predicted NAD+ binding models.

AB - The nuclear protein poly (ADP-ribose) polymerase-1 (PARP-1) plays an important role in the signaling and repair of DNA. PARP-1 catalyses the covalent binding of poly (ADP-ribose) polymers to its subunit, as well as to other acceptor proteins, using NAD+ as a donor of ADP-ribose. Inhibitors of poly (ADP-ribose) polymerase have been shown to be effective in improving radiation therapy and chemotherapy of cancer in clinical testing. Development of new poly (ADP-ribose) polymerase-1 inhibitors based on derivatives of natural compounds such as NAD+ represents a novel and promising strategy. The structure of the complex of human poly (ADP-ribose) polymerase-1 with NAD+ can be a starting point for the rational design of small molecule inhibitors based on NAD+ derivatives. Moreover, there is no crystal structure of the complex of poly (ADP-ribose) polymerase-1 with nicotinamide adenine dinucleotide (NAD+) available yet. In this work, using molecular modeling approaches, we have predicted NAD+ binding modes to PARP-1 at the donor binding site of the catalytic domain. Using structures of PARP-1 homologs in a complex with NAD+, we predict the pharmacophore restraints of NAD+ binding to PARP-1. Based on the clustering of PARP-1 conformations in a complex with cocrystallized inhibitors and the predicted pharmacophore restraints, we propose several possible models of NAD+ binding to PARP-1 at the donor binding site of the catalytic domain. According to the predicted models, two conformations for the pyrophosphate group of NAD+ in complex with PARP-1 at the donor binding site are possible. The proposed models of NAD+ binding to PARP-1 can be validated by the quantitative structure–activity analysis of NAD+ derivatives. We designed two NAD+ derivatives, which can be used to validate the predicted NAD+ binding models.

KW - NAD+

KW - PARP-1

KW - structural model

UR - http://www.scopus.com/inward/record.url?scp=85027977858&partnerID=8YFLogxK

U2 - 10.1134/S2079059717050070

DO - 10.1134/S2079059717050070

M3 - Article

AN - SCOPUS:85027977858

VL - 7

SP - 574

EP - 579

JO - Russian Journal of Genetics: Applied Research

JF - Russian Journal of Genetics: Applied Research

SN - 2079-0597

IS - 5

ER -