Stability study of the antiviral agent camphecene in dried blood spots at different temperatures

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Stability study of the antiviral agent camphecene in dried blood spots at different temperatures. / Okhina, Alina A.; Rogachev, Artem D.; Yarovaya, Olga I. и др.

в: Drug Testing and Analysis, Том 13, № 10, 10.2021, стр. 1797-1802.

Результаты исследований: Научные публикации в периодических изданиях › письмо/краткое сообщение › Рецензирование

BibTeX

@article{bea70414358845c08a6d1e5da715f791,

title = "Stability study of the antiviral agent camphecene in dried blood spots at different temperatures",

abstract = "In this study, an optimized procedure of sample preparation for quantitative determination of the antiviral agent camphecene in dried rat blood spots was developed. It has been shown that when using methanol containing 0.1% HCOOH as an extractant, the recovery of the substance increases in comparison with the previously developed method. In addition to this, there is no need to dilute the obtained solutions with water for the analysis of the sample by high-performance liquid chromatography (HPLC) on a column with a reversed-phase sorbent. By using the developed method, the stability of samples of dried rat blood spots containing camphecene in different concentrations at different temperatures was studied. It was found that while the samples were stored at room temperature, apparently, desorption of the substance occurs leading to a loss of more than 15% of its initial amount after 5–10 days. Lowering the temperature increases the stability of samples and their storage at −70°C is possible for 4 weeks.",

keywords = "antiviral agents, camphecene, dried blood spots, sample preparation, stability",

author = "Okhina, {Alina A.} and Rogachev, {Artem D.} and Yarovaya, {Olga I.} and Pokrovsky, {Andrey G.} and Salakhutdinov, {Nariman F.}",

note = "Funding Information: The authors would like to thank the Ministry of Science and Higher Education of the Russian Federation for financial support of this study (project registration number AAAA‐A21‐121011490014‐4). Publisher Copyright: {\textcopyright} 2021 John Wiley & Sons, Ltd.",

year = "2021",

month = oct,

doi = "10.1002/dta.3148",

language = "English",

volume = "13",

pages = "1797--1802",

journal = "Drug Testing and Analysis",

issn = "1942-7603",

publisher = "John Wiley and Sons Ltd",

number = "10",

}

RIS

TY - JOUR

T1 - Stability study of the antiviral agent camphecene in dried blood spots at different temperatures

AU - Okhina, Alina A.

AU - Rogachev, Artem D.

AU - Yarovaya, Olga I.

AU - Pokrovsky, Andrey G.

AU - Salakhutdinov, Nariman F.

N1 - Funding Information: The authors would like to thank the Ministry of Science and Higher Education of the Russian Federation for financial support of this study (project registration number AAAA‐A21‐121011490014‐4). Publisher Copyright: © 2021 John Wiley & Sons, Ltd.

PY - 2021/10

Y1 - 2021/10

N2 - In this study, an optimized procedure of sample preparation for quantitative determination of the antiviral agent camphecene in dried rat blood spots was developed. It has been shown that when using methanol containing 0.1% HCOOH as an extractant, the recovery of the substance increases in comparison with the previously developed method. In addition to this, there is no need to dilute the obtained solutions with water for the analysis of the sample by high-performance liquid chromatography (HPLC) on a column with a reversed-phase sorbent. By using the developed method, the stability of samples of dried rat blood spots containing camphecene in different concentrations at different temperatures was studied. It was found that while the samples were stored at room temperature, apparently, desorption of the substance occurs leading to a loss of more than 15% of its initial amount after 5–10 days. Lowering the temperature increases the stability of samples and their storage at −70°C is possible for 4 weeks.

AB - In this study, an optimized procedure of sample preparation for quantitative determination of the antiviral agent camphecene in dried rat blood spots was developed. It has been shown that when using methanol containing 0.1% HCOOH as an extractant, the recovery of the substance increases in comparison with the previously developed method. In addition to this, there is no need to dilute the obtained solutions with water for the analysis of the sample by high-performance liquid chromatography (HPLC) on a column with a reversed-phase sorbent. By using the developed method, the stability of samples of dried rat blood spots containing camphecene in different concentrations at different temperatures was studied. It was found that while the samples were stored at room temperature, apparently, desorption of the substance occurs leading to a loss of more than 15% of its initial amount after 5–10 days. Lowering the temperature increases the stability of samples and their storage at −70°C is possible for 4 weeks.

KW - antiviral agents

KW - camphecene

KW - dried blood spots

KW - sample preparation

KW - stability

UR - http://www.scopus.com/inward/record.url?scp=85113459736&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/847fd203-7bd9-357c-b447-8975223f3bbf/

U2 - 10.1002/dta.3148

DO - 10.1002/dta.3148

M3 - Letter

C2 - 34448348

AN - SCOPUS:85113459736

VL - 13

SP - 1797

EP - 1802

JO - Drug Testing and Analysis

JF - Drug Testing and Analysis

SN - 1942-7603

IS - 10

ER -

ID: 34068785