TY - JOUR

T1 - Specifics of the Taxonomic Composition of Bacterial Microflora in the Respiratory Microbiome of Patients with Chronic Dust Bronchitis

AU - Paradnikova, S. A.

AU - Druzhinin, V. G.

AU - Baranova, E. D.

AU - Demenkov, P. S.

AU - Larionov, A. V.

N1 - This work was supported by the Russian Science Foundation, grant no. 18-14-00022p. Публикация для корректировки.

PY - 2023/6

Y1 - 2023/6

N2 - Chronic dust bronchitis (CDB) is one of the most common occupational diseases among employees of coal-cycle enterprises. Until recently, the lungs were considered to be sterile, but, in recent years, studies have shown that this is not the case. However, the contribution of microbiota and individual bacterial taxa to the etiology and pathogenesis of many respiratory-tract diseases remains unclear. The aim of the study to determine the taxonomic composition of the bacterial microbiota of the upper respiratory tract of patients with CDB. Sputum samples were obtained from patients with CDB (n = 22, men) and healthy auditorial donors (n = 22, men). Sequencing of variable regions V3–V4 of the 16S pRNA gene was used to study the complete taxonomic composition of the respiratory microbiome. The study revealed a significant decrease in the α diversity of bacterial microbiota in the sputum of patients with CDB compared to the Shannon index control (H = 9.795; p = 0.0017). The PERMANOVA test using a matrix of differences constructed by the Bray–Curtis method showed a significant difference in β diversity between the compared samples (pseudo-F = 2.11; p = 0.002). In the sputum microbiome of patients with CDB, compared with the control, an increase in the relative abundance of bacteria of the genus Streptococcus was found (29.97 ± 14.21 vs. 18.78 ± 11.56; p = 0.006). The taxonomic profile of the respiratory microbiome in the study group of patients with CDB differs from that of healthy people. An increase in the relative abundance of bacteria of the genus Streptococcus in the sputum microbiome of patients with CDB suggests a general dysbiotic process with the accentuation of one dominant genus of microorganisms in this pulmonary pathology.

AB - Chronic dust bronchitis (CDB) is one of the most common occupational diseases among employees of coal-cycle enterprises. Until recently, the lungs were considered to be sterile, but, in recent years, studies have shown that this is not the case. However, the contribution of microbiota and individual bacterial taxa to the etiology and pathogenesis of many respiratory-tract diseases remains unclear. The aim of the study to determine the taxonomic composition of the bacterial microbiota of the upper respiratory tract of patients with CDB. Sputum samples were obtained from patients with CDB (n = 22, men) and healthy auditorial donors (n = 22, men). Sequencing of variable regions V3–V4 of the 16S pRNA gene was used to study the complete taxonomic composition of the respiratory microbiome. The study revealed a significant decrease in the α diversity of bacterial microbiota in the sputum of patients with CDB compared to the Shannon index control (H = 9.795; p = 0.0017). The PERMANOVA test using a matrix of differences constructed by the Bray–Curtis method showed a significant difference in β diversity between the compared samples (pseudo-F = 2.11; p = 0.002). In the sputum microbiome of patients with CDB, compared with the control, an increase in the relative abundance of bacteria of the genus Streptococcus was found (29.97 ± 14.21 vs. 18.78 ± 11.56; p = 0.006). The taxonomic profile of the respiratory microbiome in the study group of patients with CDB differs from that of healthy people. An increase in the relative abundance of bacteria of the genus Streptococcus in the sputum microbiome of patients with CDB suggests a general dysbiotic process with the accentuation of one dominant genus of microorganisms in this pulmonary pathology.

KW - 16s RNA

KW - Streptococcus

KW - chronic dust bronchitis

KW - lung microbiome

KW - sputum

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85170673688&origin=inward&txGid=afddb78ee4deafe18acc6e4cc9a0bdcc

UR - https://www.mendeley.com/catalogue/1bb24d87-6bed-3139-8355-e02ba1191653/

U2 - 10.3103/S0891416823020076

DO - 10.3103/S0891416823020076

M3 - Article

VL - 38

SP - 70

EP - 78

JO - Molecular Genetics, Microbiology and Virology

JF - Molecular Genetics, Microbiology and Virology

SN - 0891-4168

IS - 2

ER -