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Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. / Kossinova, Olga; Malygin, Alexey; Krol, Alain и др.

Methods in Molecular Biology. ред. / L Chavatte. Том 1661 Humana Press Inc., 2018. стр. 73-92 (Methods in molecular biology (Clifton, N.J.)).

Результаты исследований: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › Рецензирование

Harvard

Kossinova, O, Malygin, A, Krol, A & Karpova, G 2018, Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. в L Chavatte (ред.), Methods in Molecular Biology. Том. 1661, Methods in molecular biology (Clifton, N.J.), Humana Press Inc., стр. 73-92. https://doi.org/10.1007/978-1-4939-7258-6_6

APA

Kossinova, O., Malygin, A., Krol, A., & Karpova, G. (2018). Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. в L. Chavatte (Ред.), Methods in Molecular Biology (Том 1661, стр. 73-92). (Methods in molecular biology (Clifton, N.J.)). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7258-6_6

Vancouver

Kossinova O, Malygin A, Krol A, Karpova G. Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. в Chavatte L, Редактор, Methods in Molecular Biology. Том 1661. Humana Press Inc. 2018. стр. 73-92. (Methods in molecular biology (Clifton, N.J.)). doi: 10.1007/978-1-4939-7258-6_6

Author

Kossinova, Olga ; Malygin, Alexey ; Krol, Alain и др. / Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. Methods in Molecular Biology. Редактор / L Chavatte. Том 1661 Humana Press Inc., 2018. стр. 73-92 (Methods in molecular biology (Clifton, N.J.)).

BibTeX

@inbook{810fa4f5f962470189ae24f694f4eff7,
title = "Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis",
abstract = "Chemical approaches are very powerful tools for investigating the molecular structure and architecture of large ribonucleoprotein complexes involving ribosomes and other components of the translation system. Application of RNA nucleotide-specific and cross-linking reagents of a broad specificity range allows the researcher to obtain information on the sites of ligand binding to the ribosome and to each other as well as on the RNA rearrangements caused by the binding. Here, we describe specific chemical approaches including chemical probing and site-directed or bifunctional reagent-mediated cross-linking, which have been used for exploring the mechanism of selenocysteine insertion into a polypeptide chain by mammalian ribosomes.",
keywords = "Chemical modification, Chemical probing, Cross-linking, Ribosome, RNA, Selenocysteine, Selenoprotein, RNA, Ribosomal/chemistry, Protein Biosynthesis, Selenocysteine/chemistry, Humans, RNA-Binding Proteins/genetics, Ribosomal Proteins/metabolism, Selenoproteins/biosynthesis, Ribosome Subunits/chemistry, Animals, Cell-Free System, Ribosomes/chemistry, Protein Binding, Ligands, CONTACTS, TRANSLATION, SELENOCYSTEINE INSERTION, SBP2, SELENIUM, UGA, RNA-BINDING PROTEIN, MESSENGER-RNAS, BIOSYNTHESIS",
author = "Olga Kossinova and Alexey Malygin and Alain Krol and Galina Karpova",
year = "2018",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-7258-6_6",
language = "English",
isbn = "978-1-4939-7257-9",
volume = "1661",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "73--92",
editor = "L Chavatte",
booktitle = "Methods in Molecular Biology",

}

RIS

TY - CHAP

T1 - Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis

AU - Kossinova, Olga

AU - Malygin, Alexey

AU - Krol, Alain

AU - Karpova, Galina

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Chemical approaches are very powerful tools for investigating the molecular structure and architecture of large ribonucleoprotein complexes involving ribosomes and other components of the translation system. Application of RNA nucleotide-specific and cross-linking reagents of a broad specificity range allows the researcher to obtain information on the sites of ligand binding to the ribosome and to each other as well as on the RNA rearrangements caused by the binding. Here, we describe specific chemical approaches including chemical probing and site-directed or bifunctional reagent-mediated cross-linking, which have been used for exploring the mechanism of selenocysteine insertion into a polypeptide chain by mammalian ribosomes.

AB - Chemical approaches are very powerful tools for investigating the molecular structure and architecture of large ribonucleoprotein complexes involving ribosomes and other components of the translation system. Application of RNA nucleotide-specific and cross-linking reagents of a broad specificity range allows the researcher to obtain information on the sites of ligand binding to the ribosome and to each other as well as on the RNA rearrangements caused by the binding. Here, we describe specific chemical approaches including chemical probing and site-directed or bifunctional reagent-mediated cross-linking, which have been used for exploring the mechanism of selenocysteine insertion into a polypeptide chain by mammalian ribosomes.

KW - Chemical modification

KW - Chemical probing

KW - Cross-linking

KW - Ribosome

KW - RNA

KW - Selenocysteine

KW - Selenoprotein

KW - RNA, Ribosomal/chemistry

KW - Protein Biosynthesis

KW - Selenocysteine/chemistry

KW - Humans

KW - RNA-Binding Proteins/genetics

KW - Ribosomal Proteins/metabolism

KW - Selenoproteins/biosynthesis

KW - Ribosome Subunits/chemistry

KW - Animals

KW - Cell-Free System

KW - Ribosomes/chemistry

KW - Protein Binding

KW - Ligands

KW - CONTACTS

KW - TRANSLATION

KW - SELENOCYSTEINE INSERTION

KW - SBP2

KW - SELENIUM

KW - UGA

KW - RNA-BINDING PROTEIN

KW - MESSENGER-RNAS

KW - BIOSYNTHESIS

UR - http://www.scopus.com/inward/record.url?scp=85029591913&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7258-6_6

DO - 10.1007/978-1-4939-7258-6_6

M3 - Chapter

C2 - 28917038

AN - SCOPUS:85029591913

SN - 978-1-4939-7257-9

VL - 1661

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 73

EP - 92

BT - Methods in Molecular Biology

A2 - Chavatte, L

PB - Humana Press Inc.

ER -

ID: 12101493