TY - JOUR

T1 - Singlet-Contrast Magnetic Resonance Imaging

T2 - Unlocking Hyperpolarization with Metabolism**

AU - Eills, J.

AU - Cavallari, E.

AU - Kircher, R.

AU - Di Matteo, G.

AU - Carrera, C.

AU - Dagys, L.

AU - Levitt, M. H.

AU - Ivanov, K. L.

AU - Aime, S.

AU - Reineri, F.

AU - Münnemann, K.

AU - Budker, D.

AU - Buntkowsky, G.

AU - Knecht, S.

N1 - Publisher Copyright: © 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

PY - 2021/3/15

Y1 - 2021/3/15

N2 - Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13C, 15N, or 129Xe due to their long spin-polarization lifetimes and the absence of a proton-background signal from water and fat in the images. Here we present a novel type of 1H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para-enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D2O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization-enhanced 1H-imaging modality presented here can allow for hyperpolarized imaging without the need for low-abundance, low-sensitivity heteronuclei.

AB - Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13C, 15N, or 129Xe due to their long spin-polarization lifetimes and the absence of a proton-background signal from water and fat in the images. Here we present a novel type of 1H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para-enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D2O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization-enhanced 1H-imaging modality presented here can allow for hyperpolarized imaging without the need for low-abundance, low-sensitivity heteronuclei.

KW - hyperpolarization

KW - MRI

KW - NMR

KW - parahydrogen

KW - singlet order

UR - http://www.scopus.com/inward/record.url?scp=85100986972&partnerID=8YFLogxK

U2 - 10.1002/anie.202014933

DO - 10.1002/anie.202014933

M3 - Article

C2 - 33340439

AN - SCOPUS:85100986972

VL - 60

SP - 6791

EP - 6798

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 12

ER -