TY - JOUR

T1 - Shedding New Light on the Hull-Pericarp Adhesion Mechanisms of Barley Grains by Transcriptomics Analysis of Isogenic NUD1 and nud1 Lines

AU - Gerasimova, Sophia V.

AU - Korotkova, Anna M.

AU - Rodrigues, Tamires de S.

AU - Vikhorev, Alexander

AU - Kolosovskaya, Ekaterina V.

AU - Vasiliev, Gennady V.

AU - Melzer, Michael

AU - Hertig, Christian W.

AU - Kumlehn, Jochen

AU - Khlestkina, Elena K.

N1 - This work was supported by a grant from the Russian Science Foundation (No. 21-66-00012).

PY - 2024/12

Y1 - 2024/12

N2 - In barley having adherent hulls, an irreversible connection between the pericarp with both palea and lemma is formed during grain maturation. A mutation in the NUDUM 1 (NUD1) gene prevents this connection and leads to the formation of barley with non-adherent hulls. A genetic model of two isogenic lines was used to elucidate the genetic mechanisms of hull adhesion: a doubled haploid line having adherent hulls and its derivative with non-adherent hulls obtained by targeted mutagenesis of the NUD1 gene. Comparative transcriptomics analysis of the grain coats was performed at two stages of development: the milk stage, when the hulls can still be easily detached from the pericarp, and the dough stage when the hull adhesion process occurs. It was shown that the main differences in the transcriptomes lie in the genes related to DNA replication and chromatin assembly, cell wall organization, and cuticle formation. Meanwhile, genes involved in lipid biosynthesis mostly show minor differences in expression between stages and genotypes and represent a limited set of active genes. Among the 3-ketoacyl-CoA synthase (KCS) genes active during grain development, candidates for key enzymes responsible for very long-chain fatty acid elongation were identified.

AB - In barley having adherent hulls, an irreversible connection between the pericarp with both palea and lemma is formed during grain maturation. A mutation in the NUDUM 1 (NUD1) gene prevents this connection and leads to the formation of barley with non-adherent hulls. A genetic model of two isogenic lines was used to elucidate the genetic mechanisms of hull adhesion: a doubled haploid line having adherent hulls and its derivative with non-adherent hulls obtained by targeted mutagenesis of the NUD1 gene. Comparative transcriptomics analysis of the grain coats was performed at two stages of development: the milk stage, when the hulls can still be easily detached from the pericarp, and the dough stage when the hull adhesion process occurs. It was shown that the main differences in the transcriptomes lie in the genes related to DNA replication and chromatin assembly, cell wall organization, and cuticle formation. Meanwhile, genes involved in lipid biosynthesis mostly show minor differences in expression between stages and genotypes and represent a limited set of active genes. Among the 3-ketoacyl-CoA synthase (KCS) genes active during grain development, candidates for key enzymes responsible for very long-chain fatty acid elongation were identified.

KW - 3-ketoacyl-CoA synthase

KW - GDSL

KW - Hordeum vulgare

KW - KCS

KW - cementing layer

KW - cuticle

KW - hulled barley

KW - naked barley

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85211947808&origin=inward&txGid=0d4078c087089c4ff961a0d0953d0ba3

UR - https://www.mendeley.com/catalogue/4586c278-81a9-3751-8582-932ba9d857e6/

U2 - 10.3390/ijms252313108

DO - 10.3390/ijms252313108

M3 - Article

C2 - 39684819

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 23

M1 - 13108

ER -