TY - JOUR

T1 - Seasonal variations and interspecific differences in metabolomes of freshwater fish tissues

T2 - Quantitative metabolomic profiles of lenses and gills

AU - Tsentalovich, Yuri P.

AU - Yanshole, Vadim V.

AU - Yanshole, Lyudmila V.

AU - Zelentsova, Ekaterina A.

AU - Melnikov, Arsenty D.

AU - Sagdeev, Renad Z.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.

AB - This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.

KW - Dissolved oxygen level

KW - Freshwater fish

KW - Mass spectrometry

KW - Metabolomics

KW - NMR spectroscopy

KW - METABOLITES

KW - ANTIOXIDANTS

KW - CATARACTS

KW - N-ACETYLHISTIDINE

KW - freshwater fish

KW - DISULFIDE

KW - MASS-SPECTROMETRY

KW - OVOTHIOL

KW - metabolomics

KW - ACETYL-L-HISTIDINE

KW - mass spectrometry

KW - SALMON SALMO-SALAR

KW - dissolved oxygen level

KW - ATLANTIC SALMON

UR - http://www.scopus.com/inward/record.url?scp=85074618950&partnerID=8YFLogxK

U2 - 10.3390/metabo9110264

DO - 10.3390/metabo9110264

M3 - Article

C2 - 31684114

AN - SCOPUS:85074618950

VL - 9

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 11

M1 - 264

ER -