TY - JOUR

T1 - Replication timing in Drosophila and its peculiarities in polytene chromosomes

AU - Kolesnikova, T. D.

AU - Antonenko, O. V.

AU - Makunin, I. V.

N1 - Publisher Copyright: © Kolesnikova T.D., Antonenko O.V., Makunin I.V., 2019.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Drosophila melanogaster is one of the popular model organisms in DNA replication studies. Since the 1960s, DNA replication of polytene chromosomes has been extensively studied by cytological methods. In the recent two decades, the progress in our understanding of DNA replication was associated with new techniques. Use of fluorescent dyes increased the resolution of cytological methods significantly. High-throughput methods allowed analysis of DNA replication on a genome scale, as well as its correlation with chromatin structure and gene activity. Precise mapping of the cytological structures of polytene chromosomes to the genome assembly allowed comparison of replication between polytene chromosomes and chromosomes of diploid cells. New features of replication characteristic for D. melanogaster were described for both diploid and polytene chromosomes. Comparison of genomic replication profiles revealed a significant similarity between Drosophila and other well-studied eukaryotic species, such as human. Early replication is often confined to intensely transcribed gene-dense regions characterized by multiple replication initiation sites. Features of DNA replication in Drosophila might be explained by a compact genome. The organization of replication in polytene chromosomes has much in common with the organization of replication in chromosomes in diploid cells. The most important feature of replication in polytene chromosomes is its low rate and the dependence of S-phase duration on many factors: external and internal, local and global. The speed of replication forks in D. melanogaster polytene chromosomes is affected by SUUR and Rif1 proteins. It is not known yet how universal the mechanisms associated with these factors are, but their study is very promising.

AB - Drosophila melanogaster is one of the popular model organisms in DNA replication studies. Since the 1960s, DNA replication of polytene chromosomes has been extensively studied by cytological methods. In the recent two decades, the progress in our understanding of DNA replication was associated with new techniques. Use of fluorescent dyes increased the resolution of cytological methods significantly. High-throughput methods allowed analysis of DNA replication on a genome scale, as well as its correlation with chromatin structure and gene activity. Precise mapping of the cytological structures of polytene chromosomes to the genome assembly allowed comparison of replication between polytene chromosomes and chromosomes of diploid cells. New features of replication characteristic for D. melanogaster were described for both diploid and polytene chromosomes. Comparison of genomic replication profiles revealed a significant similarity between Drosophila and other well-studied eukaryotic species, such as human. Early replication is often confined to intensely transcribed gene-dense regions characterized by multiple replication initiation sites. Features of DNA replication in Drosophila might be explained by a compact genome. The organization of replication in polytene chromosomes has much in common with the organization of replication in chromosomes in diploid cells. The most important feature of replication in polytene chromosomes is its low rate and the dependence of S-phase duration on many factors: external and internal, local and global. The speed of replication forks in D. melanogaster polytene chromosomes is affected by SUUR and Rif1 proteins. It is not known yet how universal the mechanisms associated with these factors are, but their study is very promising.

KW - Drosophila melanogaster

KW - Endocycle

KW - Polytene chromosome

KW - Replication initiation zone

KW - Replication origins

KW - Replication timing

KW - Rif1

KW - Supressor of UnderReplication

KW - SUUR

KW - DEVELOPMENTAL CONTROL

KW - STABLE UNITS

KW - polytene chromosome

KW - replication timing

KW - ACTIVE ORIGINS

KW - replication origins

KW - DNA-REPLICATION

KW - DEPENDENT REGULATION

KW - endocycle

KW - S-PHASE

KW - GENOME-WIDE

KW - CELL-CYCLE

KW - REPLICON CLUSTERS

KW - MELANOGASTER

KW - replication initiation zone

UR - http://www.scopus.com/inward/record.url?scp=85065036452&partnerID=8YFLogxK

U2 - 10.18699/VJ19.473

DO - 10.18699/VJ19.473

M3 - Article

AN - SCOPUS:85065036452

VL - 23

SP - 140

EP - 147

JO - Вавиловский журнал генетики и селекции

JF - Вавиловский журнал генетики и селекции

SN - 2500-0462

IS - 2

ER -