TY - JOUR

T1 - Reciprocal compatibility within the genus Pisum L. as studied in F1 hybrids

T2 - 4. Crosses within P. sativum L. subsp. elatius (Bieb.) Aschers. et Graebn.

AU - Kosterin, O. E.

AU - Bogdanova, V. S.

N1 - Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.

PY - 2021/8

Y1 - 2021/8

N2 - Five accessions representing divergent lineages of wild peas (Pisum sativum subsp. elatius) were crossed with each other in both directions and also artificially pollinated with own pollen, to evaluate reproductive barriers inside Pisum sativum which may be important for pea pre-breeding. The outcome of hybrid seeds was evaluated for each combination of crosses. Reciprocal classes of F1 hybrids were compared for pollen and seed fertility and quantitative traits including seed productivity, total biomass etc. Pollination of accession VIR320 with two accessions resulted in hybrids with drastically reduced leaves and pigmentation, resembling known cases of conflict of the nucleus and plastids. Four pairs of reciprocal F1 hybrids showed strong differences in male and female fertility, supposedly a milder manifestation of cytonuclear conflict. Male and female fertility of hybrids correlated with each other, the former appearing more convenient for evaluating genetic disturbance of gametogenesis. Only two hybrid classes showed fully fertile pollen. Some of the accessions studied have been previously reported to differ in a reciprocal translocation and their hybrids were expected to have half-sterile pollen. However accession VIR320 seems to ‘bridge’ both karyological classes showing relatively high pollen fertility in some crosses with representatives of both of them, the reasons for which is discussed. Generally, strong and usually asymmetric cases of incompatibility manifested in the drop of fertility were revealed and their pattern did not correlate with phylogenetic relatedness of the accessions. Hence, wild representatives of P. sativum can hardly be subdivided into natural biological taxa below species rank and their involvement in pea pre-breeding can be complicated by unexpected crossing barriers.

AB - Five accessions representing divergent lineages of wild peas (Pisum sativum subsp. elatius) were crossed with each other in both directions and also artificially pollinated with own pollen, to evaluate reproductive barriers inside Pisum sativum which may be important for pea pre-breeding. The outcome of hybrid seeds was evaluated for each combination of crosses. Reciprocal classes of F1 hybrids were compared for pollen and seed fertility and quantitative traits including seed productivity, total biomass etc. Pollination of accession VIR320 with two accessions resulted in hybrids with drastically reduced leaves and pigmentation, resembling known cases of conflict of the nucleus and plastids. Four pairs of reciprocal F1 hybrids showed strong differences in male and female fertility, supposedly a milder manifestation of cytonuclear conflict. Male and female fertility of hybrids correlated with each other, the former appearing more convenient for evaluating genetic disturbance of gametogenesis. Only two hybrid classes showed fully fertile pollen. Some of the accessions studied have been previously reported to differ in a reciprocal translocation and their hybrids were expected to have half-sterile pollen. However accession VIR320 seems to ‘bridge’ both karyological classes showing relatively high pollen fertility in some crosses with representatives of both of them, the reasons for which is discussed. Generally, strong and usually asymmetric cases of incompatibility manifested in the drop of fertility were revealed and their pattern did not correlate with phylogenetic relatedness of the accessions. Hence, wild representatives of P. sativum can hardly be subdivided into natural biological taxa below species rank and their involvement in pea pre-breeding can be complicated by unexpected crossing barriers.

KW - Crossability

KW - Crossing barriers

KW - Pea crop wild relatives

KW - Pisum sativum L. subsp. elatius (Bieb.) Aschers. et Graebn. s.l

KW - Reproductive compatibility

KW - Translocations

KW - Wild peas

UR - http://www.scopus.com/inward/record.url?scp=85102201515&partnerID=8YFLogxK

U2 - 10.1007/s10722-021-01151-2

DO - 10.1007/s10722-021-01151-2

M3 - Article

AN - SCOPUS:85102201515

VL - 68

SP - 2565

EP - 2590

JO - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

SN - 0925-9864

IS - 6

ER -