TY - JOUR

T1 - Raman spectroscopy of yeast cells cultured on a deuterated substrate

AU - Omelchenko, Anastasia N

AU - Okotrub, Konstantin A

AU - Surovtsev, Nikolay V

N1 - This work was supported by Russian Science Foundation (Grant No. 21-74-10108 ). Raman experiments were performed in the Multiple-access center “High resolution spectroscopy of gases and condensed matters” in IA&E SB RAS (Novosibirsk, Russia). Copyright © 2023 Elsevier B.V. All rights reserved.

PY - 2023/12/15

Y1 - 2023/12/15

N2 - Raman spectroscopy of cells cultured in a deuterated substrate is a promising approach to the characterization of mass transfer and enzymatic reactions in living cells. Here, we studied the potential of this approach using the example of yeast cells cultured under aerobic and anaerobic conditions. In our experiments, unadapted to D2O Saccharomyces cerevisiae were cultured in a medium with different concentrations of deuterium oxide and deuterated glucose. It has been shown that the addition of even 10% heavy water leads to a general decrease in the amount of lipids in cells. In the Raman spectra of cells cultured at high concentrations of D2O, additional peaks are found, which are associated with the deuteration of entire chemical groups. We observed a similar effect in the ethanol synthesized by yeast fermentation, the deuteration of which also depends on the concentration of D2O. The results on the characterization of cell deuteration turned out to be in qualitative agreement with the known estimate that aerobic metabolism is 15 times more active than ethanol fermentation. The results of our work determine new limitations and prospects for further application and development of the Raman method of spectroscopy of deuterium tags.

AB - Raman spectroscopy of cells cultured in a deuterated substrate is a promising approach to the characterization of mass transfer and enzymatic reactions in living cells. Here, we studied the potential of this approach using the example of yeast cells cultured under aerobic and anaerobic conditions. In our experiments, unadapted to D2O Saccharomyces cerevisiae were cultured in a medium with different concentrations of deuterium oxide and deuterated glucose. It has been shown that the addition of even 10% heavy water leads to a general decrease in the amount of lipids in cells. In the Raman spectra of cells cultured at high concentrations of D2O, additional peaks are found, which are associated with the deuteration of entire chemical groups. We observed a similar effect in the ethanol synthesized by yeast fermentation, the deuteration of which also depends on the concentration of D2O. The results on the characterization of cell deuteration turned out to be in qualitative agreement with the known estimate that aerobic metabolism is 15 times more active than ethanol fermentation. The results of our work determine new limitations and prospects for further application and development of the Raman method of spectroscopy of deuterium tags.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85168412323&origin=inward&txGid=0910faf2d54f1ab5e2453e1c2752063d

U2 - 10.1016/j.saa.2023.123262

DO - 10.1016/j.saa.2023.123262

M3 - Article

C2 - 37607454

VL - 303

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

SN - 1386-1425

M1 - 123262

ER -