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Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing. / Mokrousova, V. I.; Okotrub, K. A.; Amstislavsky, S. Y. и др.

в: Cryobiology, Том 95, 08.2020, стр. 177-182.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Mokrousova, VI, Okotrub, KA, Amstislavsky, SY & Surovtsev, NV 2020, 'Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing', Cryobiology, Том. 95, стр. 177-182. https://doi.org/10.1016/j.cryobiol.2020.03.005

APA

Mokrousova, V. I., Okotrub, K. A., Amstislavsky, S. Y., & Surovtsev, N. V. (2020). Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing. Cryobiology, 95, 177-182. https://doi.org/10.1016/j.cryobiol.2020.03.005

Vancouver

Mokrousova VI, Okotrub KA, Amstislavsky SY, Surovtsev NV. Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing. Cryobiology. 2020 авг.;95:177-182. Epub 2020 март 11. doi: 10.1016/j.cryobiol.2020.03.005

Author

Mokrousova, V. I. ; Okotrub, K. A. ; Amstislavsky, S. Y. и др. / Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing. в: Cryobiology. 2020 ; Том 95. стр. 177-182.

BibTeX

@article{01903047b2c04e589d88ce2f17419057,
title = "Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing",
abstract = "Although lipid droplets are believed to play an important role in cryopreservation of mammalian embryos and oocytes, the effect of low temperatures on lipid droplets and related mechanisms of cryodamage are still obscure. Here, we provide Raman spectroscopy evidence of lipid separation inside the lipid droplets in domestic cat oocytes during slow freezing. It was shown that at −25 °C lipids coexist in two separated phase states inside lipid droplets. The scale of detected domains was a few micrometers size. We also found that under certain conditions these areas have a specific spatial distribution. Lipids with high melting temperatures are distributed near the surface of lipid droplets while fusible lipids are located deep inside. Raman spectroscopy was found to be a prospective approach to study inhomogeneity of lipid phase transition in cells and to reveal effects of this inhomogeneity on cryopreservation of biological cells.",
keywords = "Cryopreservation, Freezing, Freezing cell, Lipid droplet, Lipid phase transition, Oocyte, Raman spectroscopy, Triglyceride, MEMBRANE, BEHAVIOR, CRYOPRESERVATION, DAMAGE, IN-VITRO, PHASE-TRANSITION, SPATIAL-DISTRIBUTION, EMBRYOS",
author = "Mokrousova, {V. I.} and Okotrub, {K. A.} and Amstislavsky, {S. Y.} and Surovtsev, {N. V.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = aug,
doi = "10.1016/j.cryobiol.2020.03.005",
language = "English",
volume = "95",
pages = "177--182",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Raman spectroscopy evidence of lipid separation in domestic cat oocytes during freezing

AU - Mokrousova, V. I.

AU - Okotrub, K. A.

AU - Amstislavsky, S. Y.

AU - Surovtsev, N. V.

N1 - Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/8

Y1 - 2020/8

N2 - Although lipid droplets are believed to play an important role in cryopreservation of mammalian embryos and oocytes, the effect of low temperatures on lipid droplets and related mechanisms of cryodamage are still obscure. Here, we provide Raman spectroscopy evidence of lipid separation inside the lipid droplets in domestic cat oocytes during slow freezing. It was shown that at −25 °C lipids coexist in two separated phase states inside lipid droplets. The scale of detected domains was a few micrometers size. We also found that under certain conditions these areas have a specific spatial distribution. Lipids with high melting temperatures are distributed near the surface of lipid droplets while fusible lipids are located deep inside. Raman spectroscopy was found to be a prospective approach to study inhomogeneity of lipid phase transition in cells and to reveal effects of this inhomogeneity on cryopreservation of biological cells.

AB - Although lipid droplets are believed to play an important role in cryopreservation of mammalian embryos and oocytes, the effect of low temperatures on lipid droplets and related mechanisms of cryodamage are still obscure. Here, we provide Raman spectroscopy evidence of lipid separation inside the lipid droplets in domestic cat oocytes during slow freezing. It was shown that at −25 °C lipids coexist in two separated phase states inside lipid droplets. The scale of detected domains was a few micrometers size. We also found that under certain conditions these areas have a specific spatial distribution. Lipids with high melting temperatures are distributed near the surface of lipid droplets while fusible lipids are located deep inside. Raman spectroscopy was found to be a prospective approach to study inhomogeneity of lipid phase transition in cells and to reveal effects of this inhomogeneity on cryopreservation of biological cells.

KW - Cryopreservation

KW - Freezing

KW - Freezing cell

KW - Lipid droplet

KW - Lipid phase transition

KW - Oocyte

KW - Raman spectroscopy

KW - Triglyceride

KW - MEMBRANE

KW - BEHAVIOR

KW - CRYOPRESERVATION

KW - DAMAGE

KW - IN-VITRO

KW - PHASE-TRANSITION

KW - SPATIAL-DISTRIBUTION

KW - EMBRYOS

UR - http://www.scopus.com/inward/record.url?scp=85081662537&partnerID=8YFLogxK

U2 - 10.1016/j.cryobiol.2020.03.005

DO - 10.1016/j.cryobiol.2020.03.005

M3 - Article

C2 - 32171795

AN - SCOPUS:85081662537

VL - 95

SP - 177

EP - 182

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

ER -

ID: 23805022