TY - JOUR

T1 - Raman spectra of deuterated hydrocarbons for labeling applications

AU - Okotrub, Konstantin A.

AU - Shamaeva, Daria V.

AU - Surovtsev, Nikolay V.

N1 - Funding Information: This work was supported by Russian Science Foundation (Grant No. 19‐74‐00050). Raman experiments were performed in the Multiple‐access center “High resolution spectroscopy of gases and condensed matters” in IA&E SB RAS (Novosibirsk, Russia). Publisher Copyright: © 2021 John Wiley & Sons, Ltd.

PY - 2022/2

Y1 - 2022/2

N2 - Raman spectroscopy is used for studying the composition of cell lipidome. However, the Raman approach suffers from limited distinguish power for different lipids. In recent years, isotopically labeled molecules are increasingly used to enhance the Raman resolution of similar compounds in biological samples. Here, we present Raman study of fully deuterated stearic acid (stearic-d35), semi-deuterated oleic acid (oleic d-9), arachidonic acid with deuterated methine groups (arachidonic-d8), and their hydrogenated analogs. Polarization properties and temperature dependences of CD stretching modes were investigated to reveal the capability of the isotopically shifted Raman peaks in molecular characterization. Sensitivity of Raman peaks to order–disorder transition corresponding was demonstrated for deuterated methine, methylene, and methyl groups. It was found that the isotopically shifted Raman peaks provide information about the orientation of deuterated Raman tags and their surroundings.

AB - Raman spectroscopy is used for studying the composition of cell lipidome. However, the Raman approach suffers from limited distinguish power for different lipids. In recent years, isotopically labeled molecules are increasingly used to enhance the Raman resolution of similar compounds in biological samples. Here, we present Raman study of fully deuterated stearic acid (stearic-d35), semi-deuterated oleic acid (oleic d-9), arachidonic acid with deuterated methine groups (arachidonic-d8), and their hydrogenated analogs. Polarization properties and temperature dependences of CD stretching modes were investigated to reveal the capability of the isotopically shifted Raman peaks in molecular characterization. Sensitivity of Raman peaks to order–disorder transition corresponding was demonstrated for deuterated methine, methylene, and methyl groups. It was found that the isotopically shifted Raman peaks provide information about the orientation of deuterated Raman tags and their surroundings.

KW - arachidonic acid

KW - deuteration

KW - oleic acid

KW - Raman spectroscopy

KW - stearic acid

UR - http://www.scopus.com/inward/record.url?scp=85119009984&partnerID=8YFLogxK

U2 - 10.1002/jrs.6279

DO - 10.1002/jrs.6279

M3 - Article

AN - SCOPUS:85119009984

VL - 53

SP - 297

EP - 309

JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

SN - 0377-0486

IS - 2

ER -