Production of abzymes in th, cba, and c57bl/6 mice before and after mog treatment

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Production of abzymes in th, cba, and c57bl/6 mice before and after mog treatment : Comparing changes in cell differentiation and proliferation. / Aulova, Kseniya S.; Urusov, Andrey E.; Toporkova, Ludmila B. и др.

в: Biomolecules, Том 10, № 1, 53, 01.2020.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{c3dc40e0b35940c48d8d454a5a0ea2d6,

title = "Production of abzymes in th, cba, and c57bl/6 mice before and after mog treatment: Comparing changes in cell differentiation and proliferation",

abstract = "Till yet there is no data concerning mechanisms of autoimmune diseases development. Experimental autoimmune encephalomyelitis (EAE) prone C57BL/6 (T-and B-lymphocyte response), non-autoimmune CBA, and Th mice with T cell response were immunized with myelin oligodendrocyte glycoprotein (MOG35–55) to compare different characteristics of autoimmune reaction development. Bone marrow differentiation profiles of hematopoietic stem cells (HSCs), lymphocyte proliferation in various organs associated with the production of antibodies against DNA, myelin basic protein (MBP), and MOG, as well as abzymes hydrolyzing these antigens, were analyzed before and after immunization. Profiles of HSC differentiation [BFU-E (erythroid burst-forming unit (early erythroid colonies), CFU-E (erythroid burst-forming unit (late erythroid colonies), CFU-GM (granulocytic-macrophagic colony-forming unit), and CFU-GEMM granulocytic-erythroid-megakaryocytic-macrophagic colony-forming unit] and patterns of lymphocyte proliferation in different organs (brain, spleen, thymus, and lymph nodes) were very different for C57BL/6, CBA, and Th mice. We conclude that only C57BL/6 mice were predisposed to spontaneous and MOG-induced acceleration of EAE development. CBA mice are not prone to the development of autoimmune reactions. After immunization, Th mice demonstrate changes in several parameters similar to C57BL/6 and other to CBA mice; Th mice are more prone to developing autoimmune reactions than CBA mice. Our data may be important for understanding the combined presence in mice lymphocytes with T and B cell responses for spontaneous and induced autoimmune diseases.",

keywords = "Abzymes, C57BL/6 mice, Catalytic antibodies, CBA mice, Colony formation, Hematopoietic progenitor differentiation, Immunization with MOG, Th mice, hematopoietic progenitor differentiation, colony formation, B-CELLS, MYELIN BASIC-PROTEIN, HYDROLYZING ANTIBODIES, MULTIPLE-SCLEROSIS, catalytic antibodies, abzymes, C57BL, COLONY FORMATION, SERA, IGGS, AUTOIMMUNE, 6 mice, immunization with MOG, DNA, CATALYTIC HETEROGENEITY",

author = "Aulova, {Kseniya S.} and Urusov, {Andrey E.} and Toporkova, {Ludmila B.} and Sedykh, {Sergey E.} and Shevchenko, {Yuliya A.} and Tereshchenko, {Valery P.} and Sennikov, {Sergei V.} and Thomas Budde and Meuth, {Sven G.} and Popova, {Nelly A.} and Orlovskaya, {Irina A.} and Nevinsky, {Georgy A.}",

year = "2020",

month = jan,

doi = "10.3390/biom10010053",

language = "English",

volume = "10",

journal = "Biomolecules",

issn = "2218-273X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "1",

}

RIS

TY - JOUR

T1 - Production of abzymes in th, cba, and c57bl/6 mice before and after mog treatment

T2 - Comparing changes in cell differentiation and proliferation

AU - Aulova, Kseniya S.

AU - Urusov, Andrey E.

AU - Toporkova, Ludmila B.

AU - Sedykh, Sergey E.

AU - Shevchenko, Yuliya A.

AU - Tereshchenko, Valery P.

AU - Sennikov, Sergei V.

AU - Budde, Thomas

AU - Meuth, Sven G.

AU - Popova, Nelly A.

AU - Orlovskaya, Irina A.

AU - Nevinsky, Georgy A.

PY - 2020/1

Y1 - 2020/1

N2 - Till yet there is no data concerning mechanisms of autoimmune diseases development. Experimental autoimmune encephalomyelitis (EAE) prone C57BL/6 (T-and B-lymphocyte response), non-autoimmune CBA, and Th mice with T cell response were immunized with myelin oligodendrocyte glycoprotein (MOG35–55) to compare different characteristics of autoimmune reaction development. Bone marrow differentiation profiles of hematopoietic stem cells (HSCs), lymphocyte proliferation in various organs associated with the production of antibodies against DNA, myelin basic protein (MBP), and MOG, as well as abzymes hydrolyzing these antigens, were analyzed before and after immunization. Profiles of HSC differentiation [BFU-E (erythroid burst-forming unit (early erythroid colonies), CFU-E (erythroid burst-forming unit (late erythroid colonies), CFU-GM (granulocytic-macrophagic colony-forming unit), and CFU-GEMM granulocytic-erythroid-megakaryocytic-macrophagic colony-forming unit] and patterns of lymphocyte proliferation in different organs (brain, spleen, thymus, and lymph nodes) were very different for C57BL/6, CBA, and Th mice. We conclude that only C57BL/6 mice were predisposed to spontaneous and MOG-induced acceleration of EAE development. CBA mice are not prone to the development of autoimmune reactions. After immunization, Th mice demonstrate changes in several parameters similar to C57BL/6 and other to CBA mice; Th mice are more prone to developing autoimmune reactions than CBA mice. Our data may be important for understanding the combined presence in mice lymphocytes with T and B cell responses for spontaneous and induced autoimmune diseases.

AB - Till yet there is no data concerning mechanisms of autoimmune diseases development. Experimental autoimmune encephalomyelitis (EAE) prone C57BL/6 (T-and B-lymphocyte response), non-autoimmune CBA, and Th mice with T cell response were immunized with myelin oligodendrocyte glycoprotein (MOG35–55) to compare different characteristics of autoimmune reaction development. Bone marrow differentiation profiles of hematopoietic stem cells (HSCs), lymphocyte proliferation in various organs associated with the production of antibodies against DNA, myelin basic protein (MBP), and MOG, as well as abzymes hydrolyzing these antigens, were analyzed before and after immunization. Profiles of HSC differentiation [BFU-E (erythroid burst-forming unit (early erythroid colonies), CFU-E (erythroid burst-forming unit (late erythroid colonies), CFU-GM (granulocytic-macrophagic colony-forming unit), and CFU-GEMM granulocytic-erythroid-megakaryocytic-macrophagic colony-forming unit] and patterns of lymphocyte proliferation in different organs (brain, spleen, thymus, and lymph nodes) were very different for C57BL/6, CBA, and Th mice. We conclude that only C57BL/6 mice were predisposed to spontaneous and MOG-induced acceleration of EAE development. CBA mice are not prone to the development of autoimmune reactions. After immunization, Th mice demonstrate changes in several parameters similar to C57BL/6 and other to CBA mice; Th mice are more prone to developing autoimmune reactions than CBA mice. Our data may be important for understanding the combined presence in mice lymphocytes with T and B cell responses for spontaneous and induced autoimmune diseases.

KW - Abzymes

KW - C57BL/6 mice

KW - Catalytic antibodies

KW - CBA mice

KW - Colony formation

KW - Hematopoietic progenitor differentiation

KW - Immunization with MOG

KW - Th mice

KW - hematopoietic progenitor differentiation

KW - colony formation

KW - B-CELLS

KW - MYELIN BASIC-PROTEIN

KW - HYDROLYZING ANTIBODIES

KW - MULTIPLE-SCLEROSIS

KW - catalytic antibodies

KW - abzymes

KW - C57BL

KW - COLONY FORMATION

KW - SERA

KW - IGGS

KW - AUTOIMMUNE

KW - 6 mice

KW - immunization with MOG

KW - DNA

KW - CATALYTIC HETEROGENEITY

UR - http://www.scopus.com/inward/record.url?scp=85077519786&partnerID=8YFLogxK

U2 - 10.3390/biom10010053

DO - 10.3390/biom10010053

M3 - Article

C2 - 31905713

AN - SCOPUS:85077519786

VL - 10

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 1

M1 - 53

ER -

ID: 23102167

Production of abzymes in th, cba, and c57bl/6 mice before and after mog treatment: Comparing changes in cell differentiation and proliferation

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