Standard

Primary glioma cell culture: from monolayer to organoids. / Pristyazhnyuk, I E; Stupak, E V; Stupak, V V и др.

в: Protoplasma, 15.11.2025.

Результаты исследований: Научные публикации в периодических изданиях › обзорная статья › Рецензирование

Harvard

Pristyazhnyuk, IE, Stupak, EV, Stupak, VV & Menzorov, AG 2025, 'Primary glioma cell culture: from monolayer to organoids', Protoplasma. https://doi.org/10.1007/s00709-025-02137-y

APA

Pristyazhnyuk, I. E., Stupak, E. V., Stupak, V. V., & Menzorov, A. G. (2025). Primary glioma cell culture: from monolayer to organoids. Protoplasma. https://doi.org/10.1007/s00709-025-02137-y

Vancouver

Pristyazhnyuk IE, Stupak EV, Stupak VV, Menzorov AG. Primary glioma cell culture: from monolayer to organoids. Protoplasma. 2025 нояб. 15. doi: 10.1007/s00709-025-02137-y

Author

Pristyazhnyuk, I E ; Stupak, E V ; Stupak, V V и др. / Primary glioma cell culture: from monolayer to organoids. в: Protoplasma. 2025.

BibTeX

@article{0f4bf801c0e5458e8a443224e57a3f94,
title = "Primary glioma cell culture: from monolayer to organoids",
abstract = "Gliomas are the most frequent tumors of the central nervous system, with an extremely low efficiency of treatment. Primary glioma cell cultures may provide an in vitro model for studying these tumors and the development of therapeutic approaches. In this review, we assess different factors that may contribute to glioma malignancy, such as the presence of glioma stem cells, cellular heterogeneity, and selection for specific genotypes. We discuss approaches for primary glioma cell culture establishment and the role of particular components of the cultivation media: culture in monolayer, neurospheres, and glioblastoma organoids; the influence of serum, growth factors, and surface coating; and the presence of glioma stem cells. Different cell culture protocols have various drawbacks - loss of the parental tumor cellular composition, loss of glioma stem cells, or loss of the glioma microenvironment. We argue that to produce primary glioma cell culture, researchers shall use a combination of standardized protocols: serum-free neurosphere culture, serum-based monolayer culture, and glioblastoma organoids.",
author = "Pristyazhnyuk, {I E} and Stupak, {E V} and Stupak, {V V} and Menzorov, {A G}",
note = "This work was supported by the grant of the state program of the “Sirius” Federal Territory “Scientific and technological development of the {\textquoteleft}Sirius{\textquoteright} Federal Territory” (Agreement No. 26–03, 27.09.2024).",
year = "2025",
month = nov,
day = "15",
doi = "10.1007/s00709-025-02137-y",
language = "English",
journal = "Protoplasma",
issn = "0033-183X",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Primary glioma cell culture: from monolayer to organoids

AU - Pristyazhnyuk, I E

AU - Stupak, E V

AU - Stupak, V V

AU - Menzorov, A G

N1 - This work was supported by the grant of the state program of the “Sirius” Federal Territory “Scientific and technological development of the ‘Sirius’ Federal Territory” (Agreement No. 26–03, 27.09.2024).

PY - 2025/11/15

Y1 - 2025/11/15

N2 - Gliomas are the most frequent tumors of the central nervous system, with an extremely low efficiency of treatment. Primary glioma cell cultures may provide an in vitro model for studying these tumors and the development of therapeutic approaches. In this review, we assess different factors that may contribute to glioma malignancy, such as the presence of glioma stem cells, cellular heterogeneity, and selection for specific genotypes. We discuss approaches for primary glioma cell culture establishment and the role of particular components of the cultivation media: culture in monolayer, neurospheres, and glioblastoma organoids; the influence of serum, growth factors, and surface coating; and the presence of glioma stem cells. Different cell culture protocols have various drawbacks - loss of the parental tumor cellular composition, loss of glioma stem cells, or loss of the glioma microenvironment. We argue that to produce primary glioma cell culture, researchers shall use a combination of standardized protocols: serum-free neurosphere culture, serum-based monolayer culture, and glioblastoma organoids.

AB - Gliomas are the most frequent tumors of the central nervous system, with an extremely low efficiency of treatment. Primary glioma cell cultures may provide an in vitro model for studying these tumors and the development of therapeutic approaches. In this review, we assess different factors that may contribute to glioma malignancy, such as the presence of glioma stem cells, cellular heterogeneity, and selection for specific genotypes. We discuss approaches for primary glioma cell culture establishment and the role of particular components of the cultivation media: culture in monolayer, neurospheres, and glioblastoma organoids; the influence of serum, growth factors, and surface coating; and the presence of glioma stem cells. Different cell culture protocols have various drawbacks - loss of the parental tumor cellular composition, loss of glioma stem cells, or loss of the glioma microenvironment. We argue that to produce primary glioma cell culture, researchers shall use a combination of standardized protocols: serum-free neurosphere culture, serum-based monolayer culture, and glioblastoma organoids.

U2 - 10.1007/s00709-025-02137-y

DO - 10.1007/s00709-025-02137-y

M3 - Review article

C2 - 41240070

JO - Protoplasma

JF - Protoplasma

SN - 0033-183X

ER -

ID: 72237890