Prevalence Rates of the Endosymbiotic Bacterium of the Wolbachia Genus in Natural Populations of Ostrinia nubilalis and Ostrinia scapulalis (Lepidoptera

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Prevalence Rates of the Endosymbiotic Bacterium of the Wolbachia Genus in Natural Populations of Ostrinia nubilalis and Ostrinia scapulalis (Lepidoptera : Pyraloidea: Crambidae) in Southwestern Russia. / Tokarev, Yu S.; Yudina, M. A.; Malysh, J. M. и др.

в: Russian Journal of Genetics: Applied Research, Том 8, № 2, 01.07.2018, стр. 172-177.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{8010c7f410b9491daaf2b7324cb379fa,

title = "Prevalence Rates of the Endosymbiotic Bacterium of the Wolbachia Genus in Natural Populations of Ostrinia nubilalis and Ostrinia scapulalis (Lepidoptera: Pyraloidea: Crambidae) in Southwestern Russia",

abstract = "Analysis of the prevalence rates of the endosymbiotic bacterium of the Wolbachia genus in the populations of corn borers of the Ostrinia genus was carried out. Samples of genomic DNA were extracted from field-collected larvae. The PCR analysis for the presence of Wolbachia infection was performed independently using three diagnostic loci: 16SrRNA, gatB, and fbpA. A positive signal for Wolbachia was obtained for 13.5% of the Ostrinia nubilalis larvae (N = 141) and 31.9% of the Ostrinia scapulalis larvae (N = 138). The infection rate ranged from 2.9% (N = 34) to 65.8% (N = 38) in different geographical populations. At the same time, a significantly higher infection rate of O. scapulalis than O. nubilalis was observed in three out of four habitats. It was also found that the Wolbachia infection was more common for O. scapulalis individuals feeding on hemp than for those collected from mugwort.",

keywords = "corn borers, forage plants, Lepidoptera, molecular genetics detection, Wolbachia",

author = "Tokarev, {Yu S.} and Yudina, {M. A.} and Malysh, {J. M.} and Bykov, {R. A.} and Frolov, {A. N.} and Grushevaya, {I. V.} and Ilinsky, {Yu Yu}",

note = "Publisher Copyright: {\textcopyright} 2018, Pleiades Publishing, Ltd.",

year = "2018",

month = jul,

day = "1",

doi = "10.1134/S2079059718020119",

language = "English",

volume = "8",

pages = "172--177",

journal = "Russian Journal of Genetics: Applied Research",

issn = "2079-0597",

publisher = "Maik Nauka Publishing / Springer SBM",

number = "2",

}

RIS

TY - JOUR

T1 - Prevalence Rates of the Endosymbiotic Bacterium of the Wolbachia Genus in Natural Populations of Ostrinia nubilalis and Ostrinia scapulalis (Lepidoptera

T2 - Pyraloidea: Crambidae) in Southwestern Russia

AU - Tokarev, Yu S.

AU - Yudina, M. A.

AU - Malysh, J. M.

AU - Bykov, R. A.

AU - Frolov, A. N.

AU - Grushevaya, I. V.

AU - Ilinsky, Yu Yu

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Analysis of the prevalence rates of the endosymbiotic bacterium of the Wolbachia genus in the populations of corn borers of the Ostrinia genus was carried out. Samples of genomic DNA were extracted from field-collected larvae. The PCR analysis for the presence of Wolbachia infection was performed independently using three diagnostic loci: 16SrRNA, gatB, and fbpA. A positive signal for Wolbachia was obtained for 13.5% of the Ostrinia nubilalis larvae (N = 141) and 31.9% of the Ostrinia scapulalis larvae (N = 138). The infection rate ranged from 2.9% (N = 34) to 65.8% (N = 38) in different geographical populations. At the same time, a significantly higher infection rate of O. scapulalis than O. nubilalis was observed in three out of four habitats. It was also found that the Wolbachia infection was more common for O. scapulalis individuals feeding on hemp than for those collected from mugwort.

AB - Analysis of the prevalence rates of the endosymbiotic bacterium of the Wolbachia genus in the populations of corn borers of the Ostrinia genus was carried out. Samples of genomic DNA were extracted from field-collected larvae. The PCR analysis for the presence of Wolbachia infection was performed independently using three diagnostic loci: 16SrRNA, gatB, and fbpA. A positive signal for Wolbachia was obtained for 13.5% of the Ostrinia nubilalis larvae (N = 141) and 31.9% of the Ostrinia scapulalis larvae (N = 138). The infection rate ranged from 2.9% (N = 34) to 65.8% (N = 38) in different geographical populations. At the same time, a significantly higher infection rate of O. scapulalis than O. nubilalis was observed in three out of four habitats. It was also found that the Wolbachia infection was more common for O. scapulalis individuals feeding on hemp than for those collected from mugwort.

KW - corn borers

KW - forage plants

KW - Lepidoptera

KW - molecular genetics detection

KW - Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=85050000805&partnerID=8YFLogxK

U2 - 10.1134/S2079059718020119

DO - 10.1134/S2079059718020119

M3 - Article

AN - SCOPUS:85050000805

VL - 8

SP - 172

EP - 177

JO - Russian Journal of Genetics: Applied Research

JF - Russian Journal of Genetics: Applied Research

SN - 2079-0597

IS - 2

ER -

ID: 14882368

Prevalence Rates of the Endosymbiotic Bacterium of the Wolbachia Genus in Natural Populations of Ostrinia nubilalis and Ostrinia scapulalis (Lepidoptera: Pyraloidea: Crambidae) in Southwestern Russia

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