Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Preservation of Mechanical and Morphological Properties of Porcine Cardiac Outflow Vessels after Decellularization and Wet Storage. / Sergeevichev, David; Vasiliyeva, Maria; Kuznetsova, Elena и др.
в: Biomimetics (Basel, Switzerland), Том 8, № 3, 315, 17.07.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Preservation of Mechanical and Morphological Properties of Porcine Cardiac Outflow Vessels after Decellularization and Wet Storage
AU - Sergeevichev, David
AU - Vasiliyeva, Maria
AU - Kuznetsova, Elena
AU - Chelobanov, Boris
N1 - Funding: This work was carried out within the state assignment of the Ministry of Health of the Russian Federation (theme # 121031300224-1).
PY - 2023/7/17
Y1 - 2023/7/17
N2 - Widely used storage methods, including freezing or chemical modification, preserve the sterility of biological tissues but degrade the mechanical properties of materials used to make heart valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3-1.6 times in the longitudinal direction after decellularization only. The microstructures of the tested specimens showed no differences before and after wet storage. Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks does not significantly affect the strength and elastic properties of the material. The wet storage protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.
AB - Widely used storage methods, including freezing or chemical modification, preserve the sterility of biological tissues but degrade the mechanical properties of materials used to make heart valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3-1.6 times in the longitudinal direction after decellularization only. The microstructures of the tested specimens showed no differences before and after wet storage. Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks does not significantly affect the strength and elastic properties of the material. The wet storage protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85166308467&origin=inward&txGid=e4cc77b189044951a984b0c72f139aba
U2 - 10.3390/biomimetics8030315
DO - 10.3390/biomimetics8030315
M3 - Article
C2 - 37504203
VL - 8
JO - Biomimetics (Basel, Switzerland)
JF - Biomimetics (Basel, Switzerland)
SN - 2313-7673
IS - 3
M1 - 315
ER -
ID: 53251330