TY - JOUR

T1 - Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier

AU - Titov, Sergei E.

AU - Kozorezova, Evgeniya S.

AU - Demenkov, Pavel S.

AU - Veryaskina, Yulia A.

AU - Kuznetsova, Irina V.

AU - Vorobyev, Sergey L.

AU - Chernikov, Roman A.

AU - Sleptsov, Ilya V.

AU - Timofeeva, Nataliya I.

AU - Ivanov, Mikhail K.

N1 - Funding Information: Funding: This research was funded by the Russian Science Foundation, project No. 20-14-00074. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/1/11

Y1 - 2021/1/11

N2 - In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized HMGA2 mRNA level; normalized microRNA-146b,-221, and-375 levels; mitochondrialto-nuclear DNA ratio; and BRAFV600E mutation) in cytological preparations by quantitative PCR. In the present study, we aimed to estimate the specificity of the typing of different thyroid tumors by the proposed method. Fine-needle aspiration cytological preparations from 278 patients were used. The histological diagnosis was known for each sample. The positive and negative predictive values of the method assessed in this study were, respectively, 100% and 98% for papillary thyroid carcinoma (n = 63), 100% and 100% for medullary thyroid carcinoma (n = 19), 43.5% and 98% for follicular carcinoma (n = 15), and 86% and 100% for Hürthle cell carcinoma (n = 6). Thus, we demonstrate that the diagnostic panel, including the analysis of microRNA expression, mRNA expression, the BRAFV600E mutation, and the mitochondrial-to-nuclear DNA ratio, allows the highly accurate identification of papillary thyroid carcinoma, medullary thyroid carcinoma, and Hürthle cell carcinoma but not malignant follicular tumors (positive predictive value was below 50%).

AB - In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized HMGA2 mRNA level; normalized microRNA-146b,-221, and-375 levels; mitochondrialto-nuclear DNA ratio; and BRAFV600E mutation) in cytological preparations by quantitative PCR. In the present study, we aimed to estimate the specificity of the typing of different thyroid tumors by the proposed method. Fine-needle aspiration cytological preparations from 278 patients were used. The histological diagnosis was known for each sample. The positive and negative predictive values of the method assessed in this study were, respectively, 100% and 98% for papillary thyroid carcinoma (n = 63), 100% and 100% for medullary thyroid carcinoma (n = 19), 43.5% and 98% for follicular carcinoma (n = 15), and 86% and 100% for Hürthle cell carcinoma (n = 6). Thus, we demonstrate that the diagnostic panel, including the analysis of microRNA expression, mRNA expression, the BRAFV600E mutation, and the mitochondrial-to-nuclear DNA ratio, allows the highly accurate identification of papillary thyroid carcinoma, medullary thyroid carcinoma, and Hürthle cell carcinoma but not malignant follicular tumors (positive predictive value was below 50%).

KW - HMGA2

KW - MicroRNA

KW - Mitochondrial DNA

KW - Molecular marker

KW - Parathyroid adenoma

KW - Preoperative diagnosis

KW - Thyroid carcinoma

KW - mitochondrial DNA

KW - preoperative diagnosis

KW - parathyroid adenoma

KW - thyroid carcinoma

KW - molecular marker

KW - microRNA

UR - http://www.scopus.com/inward/record.url?scp=85099119031&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/f1c56294-858c-3d35-b29e-14078d52d655/

U2 - 10.3390/cancers13020237

DO - 10.3390/cancers13020237

M3 - Article

C2 - 33440616

AN - SCOPUS:85099119031

VL - 13

SP - 1

EP - 17

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 2

M1 - 237

ER -