Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Plasma exosomes of patients with breast and ovarian tumors contain an inactive 20s proteasome. / Yunusova, Natalia; Kolegova, Elena; Sereda, Elena и др.
в: Molecules, Том 26, № 22, 6965, 01.11.2021.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Plasma exosomes of patients with breast and ovarian tumors contain an inactive 20s proteasome
AU - Yunusova, Natalia
AU - Kolegova, Elena
AU - Sereda, Elena
AU - Kolomiets, Larisa
AU - Villert, Alisa
AU - Patysheva, Marina
AU - Rekeda, Irina
AU - Grigor’eva, Alina
AU - Tarabanovskaya, Natalia
AU - Kondakova, Irina
AU - Tamkovich, Svetlana
N1 - Plasma exosomes of patients with breast and ovarian tumors contain an inactive 20s proteasome / N. Yunusova, E. Kolegova, E. Sereda [et al.] // Molecules. – 2021. – Vol. 26. – No 22. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Exosomes are directly involved in governing of physiological and pathological conditions of an organism through the transfer of information from producing to receiving cells. It can be assumed that exosomes are one of the key players of tumor dissemination since they are very stable and small enough to penetrate from various tissues into biological fluids and then back, thus interacting with tissue target cells. We evaluated the enzymatic activity and the level of 20S proteasome in tissue and exosomes of healthy females (n = 39) and patients with ovarian (n = 50) and breast (n = 108) tumors to reveal the critical role of exosomal cargo in the mediation of different types of metastases. Exosomes from plasma and ascites were isolated and characterized in according to International Society for Extracellular Vesicles guidelines. The level of 20S proteasome in tissue and exosomes was determined using Western blot analysis. Chymotrypsin-and caspase-like (ChTL and CL, respectively) peptidase activities of the proteasomes were determined using fluorogenic Suc-LLVY-AMC and Cbz-LLG-AMC substrates, respectively. We observed increased levels of 20S proteasome in ovarian cancer tissue and luminal B subtype breast cancer tissue as well as in plasma exosomes from cancer patients. Moreover, the level of the 20S proteasome in plasma exosomes and ascites exosomes in patients with ovarian tumors is comparable and higher in ovarian cancer patients with low volume ascites than in patients with moderate and high-volume ascites. We also found increased ChTL and CL activities in breast cancer and ovarian cancer tissues, as well as in peritoneal metastases in ovarian cancer, while proteasomal activity in exosomes from plasma of healthy females and all patients, as well as from ascites of ovarian tumor patients were lower than detection limit of assay. Thus, regardless of the type of tumor metastasis (lymphogenous or peritoneal), the exosomes of cancer patients were characterized by an increased level of 20S proteasome, which do not exhibit enzymatic activity.
AB - Exosomes are directly involved in governing of physiological and pathological conditions of an organism through the transfer of information from producing to receiving cells. It can be assumed that exosomes are one of the key players of tumor dissemination since they are very stable and small enough to penetrate from various tissues into biological fluids and then back, thus interacting with tissue target cells. We evaluated the enzymatic activity and the level of 20S proteasome in tissue and exosomes of healthy females (n = 39) and patients with ovarian (n = 50) and breast (n = 108) tumors to reveal the critical role of exosomal cargo in the mediation of different types of metastases. Exosomes from plasma and ascites were isolated and characterized in according to International Society for Extracellular Vesicles guidelines. The level of 20S proteasome in tissue and exosomes was determined using Western blot analysis. Chymotrypsin-and caspase-like (ChTL and CL, respectively) peptidase activities of the proteasomes were determined using fluorogenic Suc-LLVY-AMC and Cbz-LLG-AMC substrates, respectively. We observed increased levels of 20S proteasome in ovarian cancer tissue and luminal B subtype breast cancer tissue as well as in plasma exosomes from cancer patients. Moreover, the level of the 20S proteasome in plasma exosomes and ascites exosomes in patients with ovarian tumors is comparable and higher in ovarian cancer patients with low volume ascites than in patients with moderate and high-volume ascites. We also found increased ChTL and CL activities in breast cancer and ovarian cancer tissues, as well as in peritoneal metastases in ovarian cancer, while proteasomal activity in exosomes from plasma of healthy females and all patients, as well as from ascites of ovarian tumor patients were lower than detection limit of assay. Thus, regardless of the type of tumor metastasis (lymphogenous or peritoneal), the exosomes of cancer patients were characterized by an increased level of 20S proteasome, which do not exhibit enzymatic activity.
KW - 20S proteasome
KW - Breast cancer
KW - Exosomes
KW - Ovarian cancer
KW - Plasma
KW - Tissue
KW - Breast Neoplasms/blood
KW - Proteasome Endopeptidase Complex/metabolism
KW - Humans
KW - Exosomes/metabolism
KW - Proteolysis
KW - Ovarian Neoplasms/blood
KW - Adult
KW - Female
UR - http://www.scopus.com/inward/record.url?scp=85119898266&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=47536092
U2 - 10.3390/molecules26226965
DO - 10.3390/molecules26226965
M3 - Article
C2 - 34834058
AN - SCOPUS:85119898266
VL - 26
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 22
M1 - 6965
ER -
ID: 34865877