Standard

Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs. / Dyudeeva, E. S.; Kupryushkin, M. S.; Lomzov, A. A. и др.

в: Russian Journal of Bioorganic Chemistry, Том 45, № 6, 01.11.2019, стр. 709-718.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Dyudeeva, ES, Kupryushkin, MS, Lomzov, AA, Pyshnaya, IA & Pyshnyi, DV 2019, 'Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs', Russian Journal of Bioorganic Chemistry, Том. 45, № 6, стр. 709-718. https://doi.org/10.1134/S1068162019060153

APA

Dyudeeva, E. S., Kupryushkin, M. S., Lomzov, A. A., Pyshnaya, I. A., & Pyshnyi, D. V. (2019). Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs. Russian Journal of Bioorganic Chemistry, 45(6), 709-718. https://doi.org/10.1134/S1068162019060153

Vancouver

Dyudeeva ES, Kupryushkin MS, Lomzov AA, Pyshnaya IA, Pyshnyi DV. Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs. Russian Journal of Bioorganic Chemistry. 2019 нояб. 1;45(6):709-718. doi: 10.1134/S1068162019060153

Author

Dyudeeva, E. S. ; Kupryushkin, M. S. ; Lomzov, A. A. и др. / Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs. в: Russian Journal of Bioorganic Chemistry. 2019 ; Том 45, № 6. стр. 709-718.

BibTeX

@article{12f5fe6ffb9a436ba91621bbcb52c91b,
title = "Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs",
abstract = "Abstract: This work describes the basic physicochemical properties of phosphoryl guanidine oligonucleotides (PGOs)—a new type of DNA analog with a partially or totally uncharged backbone. Replacement of the negatively charged oxygen atom with an electroneutral 1,3-dimethylimidazolidine-2-imine (DMI) group is shown to influence the hydrophobicity of oligonucleotide, its electrophoretic mobility, and UV absorption spectra. Thermal stability of DMI-containing DNA-duplexes was studied in aqueous solutions with different ionic strength. It is determined that, in the case of low ionic strength, the presence of DMI groups significantly enhances the thermal stability of the duplex. In the case of nearly physiological condition (0.1 M NaCl, 10 mM MgCl2) or higher ionic strength, DMI groups do not affect the duplex stability or even slightly reduce it (melting temperature changes, on average, by –1.2°C per one DMI group). An important feature of totally uncharged PGO is its ability to form the duplex effectively regardless the ionic strength, even in deionized water. In addition, according to CD data, DMI groups do not disturb the double helix structure of DNA, maintaining it in the B form.",
keywords = "DNA duplex, mass spectrometry, modified oligonucleotides, nucleic acids analogs, phosphoryl guanidine, spectrophotometry, thermal stability, NUCLEIC-ACID, DNA, HYBRIDIZATION",
author = "Dyudeeva, {E. S.} and Kupryushkin, {M. S.} and Lomzov, {A. A.} and Pyshnaya, {I. A.} and Pyshnyi, {D. V.}",
note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Ltd.",
year = "2019",
month = nov,
day = "1",
doi = "10.1134/S1068162019060153",
language = "English",
volume = "45",
pages = "709--718",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",
number = "6",

}

RIS

TY - JOUR

T1 - Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs

AU - Dyudeeva, E. S.

AU - Kupryushkin, M. S.

AU - Lomzov, A. A.

AU - Pyshnaya, I. A.

AU - Pyshnyi, D. V.

N1 - Publisher Copyright: © 2019, Pleiades Publishing, Ltd.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Abstract: This work describes the basic physicochemical properties of phosphoryl guanidine oligonucleotides (PGOs)—a new type of DNA analog with a partially or totally uncharged backbone. Replacement of the negatively charged oxygen atom with an electroneutral 1,3-dimethylimidazolidine-2-imine (DMI) group is shown to influence the hydrophobicity of oligonucleotide, its electrophoretic mobility, and UV absorption spectra. Thermal stability of DMI-containing DNA-duplexes was studied in aqueous solutions with different ionic strength. It is determined that, in the case of low ionic strength, the presence of DMI groups significantly enhances the thermal stability of the duplex. In the case of nearly physiological condition (0.1 M NaCl, 10 mM MgCl2) or higher ionic strength, DMI groups do not affect the duplex stability or even slightly reduce it (melting temperature changes, on average, by –1.2°C per one DMI group). An important feature of totally uncharged PGO is its ability to form the duplex effectively regardless the ionic strength, even in deionized water. In addition, according to CD data, DMI groups do not disturb the double helix structure of DNA, maintaining it in the B form.

AB - Abstract: This work describes the basic physicochemical properties of phosphoryl guanidine oligonucleotides (PGOs)—a new type of DNA analog with a partially or totally uncharged backbone. Replacement of the negatively charged oxygen atom with an electroneutral 1,3-dimethylimidazolidine-2-imine (DMI) group is shown to influence the hydrophobicity of oligonucleotide, its electrophoretic mobility, and UV absorption spectra. Thermal stability of DMI-containing DNA-duplexes was studied in aqueous solutions with different ionic strength. It is determined that, in the case of low ionic strength, the presence of DMI groups significantly enhances the thermal stability of the duplex. In the case of nearly physiological condition (0.1 M NaCl, 10 mM MgCl2) or higher ionic strength, DMI groups do not affect the duplex stability or even slightly reduce it (melting temperature changes, on average, by –1.2°C per one DMI group). An important feature of totally uncharged PGO is its ability to form the duplex effectively regardless the ionic strength, even in deionized water. In addition, according to CD data, DMI groups do not disturb the double helix structure of DNA, maintaining it in the B form.

KW - DNA duplex

KW - mass spectrometry

KW - modified oligonucleotides

KW - nucleic acids analogs

KW - phosphoryl guanidine

KW - spectrophotometry

KW - thermal stability

KW - NUCLEIC-ACID

KW - DNA

KW - HYBRIDIZATION

UR - http://www.scopus.com/inward/record.url?scp=85078599405&partnerID=8YFLogxK

U2 - 10.1134/S1068162019060153

DO - 10.1134/S1068162019060153

M3 - Article

AN - SCOPUS:85078599405

VL - 45

SP - 709

EP - 718

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 6

ER -

ID: 23256607