TY - JOUR

T1 - PENETRATION OF SIROLIMUS AND PACLITAXEL RELEASED BY ELECTROSPUN PRODUCED DRUG ELUTING STENTS COATINGS THROUGH THE ARTERIAL WALL

AU - Nazarkina, Zhanna K.

AU - Челобанов, Борис Павлович

AU - Кузнецов, Константин

AU - Romanova, Irina V.

AU - Савостьянова, Татьяна Алексеевна

AU - Laktionov, Pavel P.

N1 - The study was supported by the grants from the Russian Science Foundation no. 18-15-00080 and State Funded Project no. 0245-2021-0007

PY - 2022/4

Y1 - 2022/4

N2 - Drug-eluting stents (DES) were designed to minimize neointima growth after angioplasty [1]. Sirolimus (SRL) and paclitaxel (PTX) are the most commonly used DES coating drugs now. It was shown that, PCL-based electrospun produced, SRL and PTX enriched matrices exhibit long-term drug release kinetic and are to be used as coatings of DES [2-4]. The retention of drug released from stent coatings by the wall of rabbit iliac artery (IAW) was studied. DES coated with matrices 5%PCL/10%HSA/3%DMSO/SRL or 5%PCL/10%HSA/3%DMSO/PTX with tritium-labeled drugs in dose of 0.9 and 0.46mg/cm2, respectively, were produced using electrospinning. Coated stents were installed into freshly obtained rabbit’s iliac artery and fixed in special device. The drug release and penetrating through IAW in PBS or in blood plasma (BP) was evaluated by radioactivity of PBS/BP. IAW retain PTX and decrese release from coating in 3-4 times during first hours and 2-2.5 times during the day. After 24 hours more than half of PTX released from the coating is retained in IAW. Even more efficient retention was observed for SRL; only 12.5% of SRL released from coating into BP pass through the IAW. Retention of drugs by IAWis more efficient when drugs were released in BP with a plateau reached in 9 hours. The retention/accumulation of drugs by IAWprovides a prolonged drug release and allows reducing the dose of drugs in stent coatings.

AB - Drug-eluting stents (DES) were designed to minimize neointima growth after angioplasty [1]. Sirolimus (SRL) and paclitaxel (PTX) are the most commonly used DES coating drugs now. It was shown that, PCL-based electrospun produced, SRL and PTX enriched matrices exhibit long-term drug release kinetic and are to be used as coatings of DES [2-4]. The retention of drug released from stent coatings by the wall of rabbit iliac artery (IAW) was studied. DES coated with matrices 5%PCL/10%HSA/3%DMSO/SRL or 5%PCL/10%HSA/3%DMSO/PTX with tritium-labeled drugs in dose of 0.9 and 0.46mg/cm2, respectively, were produced using electrospinning. Coated stents were installed into freshly obtained rabbit’s iliac artery and fixed in special device. The drug release and penetrating through IAW in PBS or in blood plasma (BP) was evaluated by radioactivity of PBS/BP. IAW retain PTX and decrese release from coating in 3-4 times during first hours and 2-2.5 times during the day. After 24 hours more than half of PTX released from the coating is retained in IAW. Even more efficient retention was observed for SRL; only 12.5% of SRL released from coating into BP pass through the IAW. Retention of drugs by IAWis more efficient when drugs were released in BP with a plateau reached in 9 hours. The retention/accumulation of drugs by IAWprovides a prolonged drug release and allows reducing the dose of drugs in stent coatings.

UR - https://www.liebertpub.com/doi/full/10.1089/ten.tea.2022.29025.abstracts

U2 - 10.1089/ten.tea.2022.29025.absrtacts

DO - 10.1089/ten.tea.2022.29025.absrtacts

M3 - Conference article

VL - 28

SP - 492

EP - 492

JO - TISSUE ENGINEERING. PART A

JF - TISSUE ENGINEERING. PART A

SN - 1937-3341

IS - S1

ER -