TY - JOUR

T1 - Optical uncaging of ADP reveals the early calcium dynamics in single, freely moving platelets

AU - Spiryova, Darya V.

AU - Vorobev, Alexei Yu

AU - Klimontov, Vadim V.

AU - Koroleva, Elena A.

AU - Moskalensky, Alexander E.

N1 - © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Platelet activation is considered to be a cornerstone in pathogenesis of cardiovascular disease. The assessment of platelet activation at the single-cell level is a promising approach for the research of platelet function in physiological and pathological conditions. Previous studies used the immobilization of platelets on the surface, which significantly alters the activation signaling. Here we show that the use of photolabile "caged" analog of ADP allows one to track the very early stage of platelet activation in single, freely moving cells. In this approach, the diffusion step and ADP receptor ligation are separated in time, and a millisecond-timescale optical pulse may trigger the activation. The technique allows us to measure the delay (lag time) between the stimulus and calcium response in platelets. We also propose a simple model function for calcium peaks, which is in good agreement with the measured data. The proposed technique and model function can be used for in-depth studies of platelet physiology.

AB - Platelet activation is considered to be a cornerstone in pathogenesis of cardiovascular disease. The assessment of platelet activation at the single-cell level is a promising approach for the research of platelet function in physiological and pathological conditions. Previous studies used the immobilization of platelets on the surface, which significantly alters the activation signaling. Here we show that the use of photolabile "caged" analog of ADP allows one to track the very early stage of platelet activation in single, freely moving cells. In this approach, the diffusion step and ADP receptor ligation are separated in time, and a millisecond-timescale optical pulse may trigger the activation. The technique allows us to measure the delay (lag time) between the stimulus and calcium response in platelets. We also propose a simple model function for calcium peaks, which is in good agreement with the measured data. The proposed technique and model function can be used for in-depth studies of platelet physiology.

KW - AGGREGATION

KW - MOBILIZATION

KW - HOMEOSTASIS

KW - SPIKING

KW - P2Y(1)

UR - http://www.scopus.com/inward/record.url?scp=85085841096&partnerID=8YFLogxK

U2 - 10.1364/BOE.392745

DO - 10.1364/BOE.392745

M3 - Article

C2 - 32637257

AN - SCOPUS:85085841096

VL - 11

SP - 3319

EP - 3330

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 6

ER -